PathFinder™ PD-1:PD-L2[Biotinylated] Inhibitor Screening Colorimetric Assay Kit (CAT#: IOK-02P083)

After binding to PD-L2 ligand, the cell signal transduction through PD-1 receptor; weakens the immune response, and is used by tumors and viruses. PD-1: PDL2 [; [Biotinylation] Inhibitor Screening Colorimetric Assay Kit is designed to screen inhibitors of the following drugs: PD-L2 ligand binds to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L2 to streptavidin-HRP. The determination only requires a few simple steps on the microtiter plate. First, coat PD-1 on it; 96-well plate. Next, incubate PD-L2 and PD-1 on the plate. Finally, treat the plate with the following method: Streptavidin-HRP, and then add a colorimetric HRP substrate to produce color, which can then be measured with a UV/Vis spectrophotometer plate reader.

Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand; attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL2[; Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of; PD-L2 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L2 for streptavidin-HRP.; Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on; a 96-well plate. Next, PD-L2 is incubated with PD-1 on the plate. Finally, the plate is treated with; streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which; can then be measured using a UV/Vis spectrophotometer microplate reader.

This kit is most useful for screening for inhibitors of PD1 binding to PD-L2.

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC