Rat CD80 ELISA Kit, Lot 21AO-186 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P097)

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

<2pg/mL

Pre-coated

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL rat CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates or serum to each empty well.

Sample 1: n=16, Mean(ng/ml): 0.28, Standard deviation: 0.015, CV(%): 5.2
Sample 2: n=16, Mean(ng/ml): 1.56, Standard deviation: 0.111, CV(%): 7.1
Sample 3: n=16, Mean(ng/ml): 2.31, Standard deviation: 0.113, CV(%): 4.9
Sample 1: n=24, Mean(ng/ml): 0.25, Standard deviation: 0.016, CV(%): 6.2
Sample 2: n=24, Mean(ng/ml): 1.72, Standard deviation: 0.138, CV(%): 8.0
Sample 3: n=24, Mean(ng/ml): 2.48, Standard deviation: 0.134, CV(%): 5.4

Avoid multiple freeze-thaw cycles.

-20 °C,4 °C

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

12 months

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

For Research Use only

B-Lymphocyte Activation Antigen B7-1 (LAB7-1)

B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; B71; Cd28l; Ly-53; Ly53; MIC17; TSA1; CD80 molecule; Cd80 molecule; CD80 antigen; CD80; Cd80

25411

G3V671

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints

A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, I39-Q248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.