Rat PVRL2 ELISA Kit, Lot 21AO-227 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P138)

The kit is designed for in vitro quantitative measurement of Rat PVRL2 in Plasma, Serum.

The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PVRL2 in Rat serum, plasma and other biological fluids

ELISA

ELISA Plate: The just opened ELISA Plate may appear water-like substance, which is normal and will not have any impact on the experiment results.
Add Sample: The interval of sample adding between the first well and the last well should not be too long, otherwise will cause different pre-incubation time, which will significantly affect the experiment's accuracy and repeatability.
Incubation: To prevent evaporation and ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.
Washing: The wash procedure is critical.
Reagent Preparation: As the volume of Detection Ab and HRP Conjugate is very small, liquid may adhere to the tube wall or tube cap when being transported. You better hand-throw it or centrifugal it for 1 minute at 1000rpm. Please pipette the solution for 4-5 times before pippeting.
Substrate: Substrate Solution is easily contaminated. Please protect it from light.

Information on standard material: The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents: Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies: The provided antibodies and their host vary in different kits. All antibodies are affinity purified.

PVRL2

Rat

Colorimetric

Sandwich ELISA

Quantitative

Plasma, Serum

This kit recognizes natural and recombinant Rat PVRL2. No significant cross-reactivity or interference between Rat PVRL2 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Rat PVRL2 and all the analogues.

No significant cross-reactivity or interference between rat Poliovirus Receptor Related Protein 2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Human bFGF and all the analogues, therefore, cross reaction may still exist.