TF-1a In Vitro Reporter Gene (β-galactosidase) Assay (CAT#: ITS-1122-YF1674)

TF-1a

Human

TF-1a is a factor-independent variant isolated from the factor-dependent TF-1 cell line. It can be used to study signal pathways involved in the spontaneous and factor-induced growth of the cells.

Erythroleukemia

Oncology

In Vitro Reporter Gene (β-galactosidase) Assay

TF-1a-cell based In Vitro Reporter Gene (β-galactosidase) Assay

Reporter gene assays have become a valuable technique for studying gene expression by both cis- and trans-acting regulatory factors. The action of a reporter gene is evaluated in a reporter gene assay and a reporter gene construct normally comprises of a target regulatory DNA sequence (usually a major in a cancer pathway) to be analyzed, sequence for the reported gene and a sequence for functional mRNA in an expression vector. The expression vector can be transfected into the desired cell type. After transfection of the vector, the effects on a drug on the expression of target gene can be evaluated. The ideal reported gene activity should detect with high sensitivity over any endogenous gene expression. Reporter gene systems permit the use of cancer pathway-specific or developmentally regulated regulatory DNA sequences as markers to analyze gene expression in specific pathways associated with molecular mechanisms of cancer.

β-galactosidase

High-throughput Screening, High Content Screening and Reporter Gene Assays