U-251 In Vitro Hydrogen Peroxide Scavenging Activity Assay (Oxidative Stress) (CAT#: ITS-1022-YF4805)

U-251

Human

The U-251 cell line was established at the Wallenberg laboratory, Uppsala, Sweden, almost more than 40 years ago along with several other cell lines derived from human gliomas, and was derived from a male patient with malignant astrocytoma.

Glioblastoma

Oncology

In Vitro Hydrogen Peroxide Scavenging Activity Assay (Oxidative Stress)

U-251-cell based In Vitro Hydrogen Peroxide Scavenging Activity Assay (Oxidative Stress)

It's a commonly used simple antioxidant assay. The ability of antioxidants to scavenge NO. and hydroxyl free radicals (OH.) is measured in NO and hydrogen peroxide scavenging assays respectively.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Antioxidant Assay