Precise quantification of ABCA1 function and expression is vital for investigating its role in atherosclerosis progression, HDL metabolism, and reverse cholesterol transport. This analysis assists in understanding disease mechanisms and evaluating potential therapeutic interventions for conditions like coronary artery disease and familial hypoalphalipoproteinemia.
As one of the targets in our singleplex biomarker assays, Creative Biolabs proudly offers cutting-edge ABCA1 analysis services. Leveraging powerful analytical platforms and the expertise of seasoned professionals, we provide comprehensive solutions for ABCA1 identification, quantification, and functional assessment, delivering the convincing results essential for your critical research endeavors.
Introduction of ABCA1
The ATP-binding cassette transporter A1 (ABCA1), a pivotal 220 kDa transmembrane protein encoded by the ABCA1 gene on human chromosome 9, is crucial for lipid metabolism. Widely expressed, ABCA1 facilitates the efflux of intracellular phospholipids and free cholesterol to apolipoprotein A-I (ApoA-I). This process is the rate-limiting step in nascent high-density lipoprotein (HDL) formation, essential for reverse cholesterol transport (RCT) that clears excess cholesterol and prevents atherosclerosis. ABCA1 also impacts plasma lipid content, microparticle formation, and cell signaling. Its mutations are strongly associated with several critical human diseases.
Fig.1 ABCA1 participate in HDL biosynthesis.1
ABCA1: A Biomarker for Tangier Disease and Familial HDL Deficiency
Tangier disease, a rare genetic disorder, manifests as severely reduced circulating HDL. This condition stems from ABCA1 gene mutations that impair its essential transport function. Normally, ABCA1 promotes nascent HDL synthesis by moving cholesterol and phospholipids to ApoA-I; however, mutations compromise this, leading to significantly reduced HDL biosynthesis. Consequently, ABCA1 is an indispensable biomarker for diagnosing and characterizing Tangier disease and other familial HDL deficiencies, enabling targeted patient management.
ABCA1 Analysis Services at Creative Biolabs
Creative Biolabs offers a first-class and reliable suite of analytical services for the precise identification and quantification of ABCA1. Our dedicated research teams and state-of-the-art R&D laboratories, strategically located worldwide, ensure swift and professional analysis of diverse targets for our global clientele. Our robust methodology encompasses both protein and gene-level investigations to provide a holistic understanding of ABCA1 status.
Our high-quality ABCA1 analysis services include, but are not limited to:
- ABCA1 Protein Level Testing
Employing a range of sensitive immunological assays such as ELISA, Lateral Flow Assay (LFA), Chemiluminescence Immunoassay (CLIA), Luminescence Enzyme Immunoassay (LEIA), and Western Blot (WB) to accurately quantify ABCA1 protein expression.
- ABCA1 Protein Sequencing
Detailed analysis of the ABCA1 protein sequence to identify specific amino acid variations or post-translational modifications.
- ABCA1 Single Gene Testing
Targeted genetic analysis of the ABCA1 gene to detect mutations, single nucleotide polymorphisms (SNPs), or other genetic variations linked to disease or altered function.
- Customized ABCA1 Analysis Services
Tailored solutions for unique research requirements, including more complex functional studies or investigations into specific disease associations such as diabetes, thrombosis, and cancer.
Service Workflow
Our commitment to efficiency and precision is reflected in our structured service workflow, designed to provide a seamless experience for our clients.
01Initial Consultation
The process begins with our scientific team collaborating with you to thoroughly understand your specific research objectives and define the most appropriate scope for your ABCA1 analysis, ensuring tailored solutions.
02Sample Submission
Clients then provide the necessary biological samples (e.g., cells, tissues, plasma, purified proteins). We offer clear guidelines to ensure proper handling and optimal sample integrity for subsequent analysis.
03Sample Processing and Analysis
Our expert scientists meticulously process and analyze these samples using advanced platforms. Strict adherence to established protocols and rigorous quality control measures guarantees high-standard and reliable data generation.
04Communication and Updates
Throughout the analysis phase, we maintain transparent communication, providing regular progress updates. This ensures you are always informed about your project's status.
05Comprehensive Report Delivery
Finally, we deliver a detailed report containing all results, methodology, clear data interpretation, and actionable recommendations, empowering your research with invaluable insights.
Applications
Cardiovascular Disease Research
Metabolic Disorder Studies
ABCA1 analysis provides critical data for exploring its connection to metabolic syndrome and Type 2 Diabetes, particularly concerning pancreatic beta-cell function and insulin production. Understanding how ABCA1 dysfunction influences glucose homeostasis opens new avenues for therapeutic development.
Neurodegenerative Disease Research
Investigating ABCA1 expression and activity in neurological samples helps elucidate its involvement in cholesterol efflux within the central nervous system, which is increasingly recognized as a factor in the pathogenesis of neurodegenerative conditions such as Alzheimer's Disease.
Drug Discovery and Development
ABCA1 analysis is fundamental for screening and evaluating compounds that modulate its activity. This includes identifying potential agonists to enhance cholesterol efflux or antagonists for specific research purposes, significantly contributing to lead optimization in pharmaceutical development.
Biomarker Identification and Validation
Our services support the discovery and validation of ABCA1 as a robust biomarker for early diagnosis, prognosis, and monitoring of treatment efficacy across various diseases, improving patient stratification and personalized medicine approaches.
Service Highlights
- Unparalleled Expertise: Our team comprises highly experienced biologists and analytical scientists with deep knowledge of lipid metabolism and ABCA1 biology, ensuring meticulous experimental design and insightful data interpretation.
- Advanced Analytical Platforms: We utilize cutting-edge technologies and robust methodologies, including state-of-the-art immunoassay systems and advanced genetic sequencing platforms, to deliver precise and reliable results.
- Tailored Solutions: Understanding that each research project is unique, we offer highly customizable service packages, adapting our comprehensive analysis capabilities to meet your specific experimental objectives and timelines.
- Global Reach and Support: With R&D laboratories and dedicated teams around the world, Creative Biolabs provides responsive and localized support to clients globally, ensuring a seamless and efficient research partnership.
FAQs
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Can you help with the interpretation of genetic variants identified in the ABCA1 gene?
Absolutely. Our team includes experts in genetics and molecular biology who can assist with the interpretation of identified ABCA1 gene variants and polymorphisms. We provide detailed reports that contextualize findings with known clinical associations, functional impacts, and population frequencies, helping you understand the potential implications of specific genetic changes for disease susceptibility or therapeutic response.
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What is the sensitivity of your cholesterol efflux assays for measuring ABCA1 activity?
Our cholesterol efflux assays are designed with high sensitivity to accurately quantify even subtle changes in ABCA1 activity. We utilize optimized cell lines and detection methods, including radiolabeled or fluorescent cholesterol analogs, to ensure robust and reproducible measurements, making them suitable for detecting both significant and nuanced modulations of ABCA1 function.
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Do you offer custom assay development for unique ABCA1-related research needs?
Yes, indeed. We specialize in providing customized solutions. If your research requires a unique approach to ABCA1 analysis not covered by our standard offerings, our R&D team is adept at developing and validating bespoke assays. This collaborative approach ensures that the analytical methods are perfectly aligned with your specific scientific objectives.
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How do you ensure the quality and reproducibility of your ABCA1 analysis results?
Quality and reproducibility are paramount at Creative Biolabs. We implement stringent quality control measures at every stage of the analytical process, from sample handling and reagent validation to instrument calibration and data analysis. Our laboratories adhere to strict GLP-compliant standards, and all assays undergo rigorous validation to ensure accuracy, precision, and consistency of results across experiments.
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Can your services distinguish between ABCA1 protein expression and its functional activity?
Yes, our comprehensive suite of services allows for this distinction. While protein level tests like ELISA or Western Blot quantify ABCA1 expression, our functional assays, such as cholesterol efflux assays and ATPase activity measurements, directly assess the protein's biological activity. This combined approach provides a more complete picture of ABCA1 status, which is crucial for understanding its role in health and disease.
With professional teams and full rigged technology platforms, Creative Biolabs is your best choice for academic research and product development. In recent years, with the in-depth learning of ABCA1, its relationship with diabetes, thrombosis, cancer and other complex diseases has been gradually reported. In this respect, we also provide customized services to meet your various research needs.
If you have any requirements for ABCA1 analysis, please do not hesitate to contact us for more information.
Reference
- Xiao, Mingming, et al. "Functional significance of cholesterol metabolism in cancer: from threat to treatment." Experimental & Molecular Medicine 55.9 (2023): 1982-1995. Distributed under Open Access license CC BY 4.0, without modification.
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