Interested in moving your program forward, but don't want to bring an assay in-house?
Creative Biolabs can help you! Our scientists will use their extensive biochemistry and immunology expertise to work with you and accelerate your Janus-activated kinase 1 (JAK1) program.
Scientific Validity
JAK1 is a member of non-receptor tyrosine kinases family which are involved in autoimmune diseases and malignancies. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It plays a critical role in metastatic cancer progression and mediates the persistent oncogenic activation of STAT3 in mammary cancer cells. Numerous studies indicated that JAK1 works as either an oncogene or a tumor suppressor under certain conditions.
JAK1 Inhibitor
JAK1 modulates a subset of proinflammatory cytokines within the JAK-STAT pathway. JAK1 inhibition may be primarily responsible for the therapeutic efficacy of pan-JAK inhibitors in immune-mediated diseases, supporting the use of selective inhibition of JAK1 as a therapeutic strategy. JAK1 loss of function mutations may have important role in the absence of response to PD-1 inhibitors due to lower ability of immune T cells to recognize tumor cells.
Fig.1 Therapeutic targets of the JAK/STAT signaling pathway.1
Upon ligand binding to cytokine receptors, JAK1 phosphorylate themselves and their associated receptors. This provides multiple binding sites for signaling proteins. Thus, JAK kinases provide important links between cytokine/hormone receptors and downstream effector proteins, and efficient study methods are in high demand.
Services at Creative Biolabs
Creative Biolabs is a leader in the antibody development industry. We have developed high-quality phospho-JAK1 antibodies which specifically recognize JAK1 when phosphorylated at special sites and does not cross-react with related kinases. The total-JAK1 antibody recognizes JAK1 proteins regardless of the phosphorylation state. By determining phosphorylated JAK1 protein in experimental model system, we can verify pathway activation in your cell lysates.
Lead Discovery Services Include
- JAK1 expression profiles determination
- Phospho-JAK1 and total JAK1 ELISA
Analysis services targeting the epitopes listed below are available.
| pTyr1034 | pTyr1035 | Phosphospecific |
| pThr246 | Total | Phosphotyrosine |
- Cell-based ELISA
Creative Biolabs provides sensitive method for detecting phospho-JAK1 directly in the cell, without making extracts or performing electrophoresis and membrane blotting. These assays are available in both colorimetric and chemiluminescent formats.
- JAK1 kinase activity assay & Inhibitor screening
- Functional assay
- Mechanism of action studies-kinetic analysis to define the mode of inhibition.
If you are interested in our analysis services, please feel free to contact us for more information. We will respond quickly to get the conversation moving and learn how we can help. We keep things discrete, confidential, and professional. Let's Discover Together.
Reference
- Hu, Xiaoyi, et al. "The JAK/STAT signaling pathway: from bench to clinic." Signal transduction and targeted therapy 6.1 (2021): 402. Distributed under Open Access license CC BY 4.0, without modification.
For Research Use Only.