In recent decades, research focuses on ATM distribution, cytokines involved in ATM regulation, ATM inhibitors and so forth. Creative Biolabs provides one-stop services with a variety of test platforms for ATM analysis. Currently, we have launched a series of biomarker analysis services to promote disease research. Here, we introduce the ATM biomarker analysis.
ATM Activation Mechanisms
ATM serine/threonine kinase (ATM) activation occurs in two different ways. The classic ATM activation pathway relies on double-strand DNA break (DSB) and MRN complexes. ATM usually exists in the nucleus as a homodimer (non-activated state), which becomes a monomer after activation to interact with the end of DNA and its substrate, and then participates in cell cycle checkpoint control, apoptotic reaction and DNA repair, etc. The other way is to form disulfide bond between two ATM monomers under oxidative stress, thus activating ATM.
ATM activity has been associated with a variety of diseases and can be monitored in real time using traditional protein analysis methods, fluorescent protein and in vitro bioluminescence techniques.
Fig.1 Mechanisms into the activation of ATM kinase.Distributed under CC BY-SA 3.0, from Wiki,
without modification.
ATM and Human Diseases
Loss of ATM protein activity in the human body leads to a polytropic neurodegenerative disease called ataxia telangiectasia (AT), which leads to immune system deficiencies, cancer susceptibility, etc.
ATM Kinase Inhibitor
Recent studies have shown that ATM can repair DNA DSBs and that targeted therapy enables tumor cells to become constitutionally dependent on ATM kinase. ATM inhibitors can clear resistant residual tumor cells, resulting in a more permeable, longer lasting effect. Some ATM inhibitors have emerged as potential candidates for cancer treatment, the following are several ATM inhibitors.
CGK733
ATM/ATR inhibitors, used in cancer research.
AZ32
An orally available ATM inhibitor that penetrates the blood-brain barrier.
CP-466722
Reversible ATM inhibitors have no effect on PI3K and PIKKs family proteins.
Ro 90-7501
Inhibit ATM phosphorylation and DNA repair.
Services Provided by Creative Biolabs
In the past few years, significant advances have been made in ATM molecular-specific protein assays, fluorescence or radiolabeling.
ATM visualization of molecular imaging technology, from the traditional quantitative protein analysis to ATM inhibitor analysis for real-time monitoring of ATM activity. Our services can be customized to suit the specific needs of our clients, popular analysis services targeting ATM include but not limited to the following:
| Cat | Service |
|---|---|
| BAS117-1 | ATM Antibody Development |
| BAS117-2 | ATM Inhibitor Screening Assay |
| BAS117-3 | ATM Inhibitory Activity Assay |
| BAS117-4 | ATM Protein Analysis |
| BAS117-5 | ATM Immunoblotting Assay |
| BAS117-6 | ATM Immunofluorescence Assay |
| BAS117-7 | ATM Immunohistochemical Assay |
| BAS117-8 | ATM Gene Analysis |
| BAS117-9 | ATM Chromatin Immunoprecipitation Analysis |
| BAS117-10 | ATM Kinase Assay |
| BAS117-11 | ATM Mass Spectrometry Analysis |
| BAS117-12 | ATM Phosphorylation Analysis |
We are committed to providing the highest quality of custom services and products at the most reasonable prices. Please feel free to contact us for more information and a formal quote.
For Research Use Only.