Chemiluminescent immunoassay is a variation of the standard enzyme immunoassay (EIA), which is a biochemical technique used in immunology. They can also be used as diagnostic tools in medicine, as well as being used in several other different industries for various applications. Creative Biolabs is a world leader in the development of ELISA based kits and provides a wide range of assay kits for specific detection of disease-related proteins. Methods based on enzyme labels have been developed for chemiluminescent immunoassays in Creative Biolabs. Our professional team is optimized to help you with high-quality and cost-effective testing service to make your project a success.

How does it work?

This technique takes advantage of the basic immunology concept of antigen binding to specific antibodies, which uses enzymatically labeled antibodies and antigens to detect the desired small biological molecules. Such antigenic molecules that can be identified in fluid samples include molecules such as peptides, proteins, and hormones. Enzymes for chemiluminescent immunoassays convert substrates into reaction products that emit photons of light instead of forming a specific color. Luminescence means that when the substance returns from the excited state to the ground state, the light is emitted by it. There are different types of luminescence, and the various forms are different in the way they reach the excited state. For chemiluminescence, it is light produced by a chemical reaction. The chemiluminescent substance can be excited by an oxidation reaction forming an intermediate. When this immediate returns to a stable ground state, the photons are released and detected by a luminescent signal instrument. The specific luminescence indicates the presence of the antigen. The number of specific biomolecules, which is being looked for and present in the sample, is based on the luminescence observed.

Fig.1 Detection method for CLIA.Fig.1 The principle of CLIA.1

Advantages of Our Chemiluminescent Immunoassays (CLIA) Platforms

Creative Biolabs has been devoted for the development of highly sensitive, more flexible, quantitative, and easy-to-use chemiluminescent immunoassays-based kits. There are several advantages of using chemiluminescence rather other types of immunoassay, including:

  • Ultra-sensitive
  • Small amounts of the biological molecule can be detected
  • Wide dynamic range
  • Linear relationship between luminous intensity and the concentration of the measured substance
  • The reactions can be enhanced with an enhancer

To assist customers for specific detection of disease-related proteins, Creative Biolabs provides a variety of ELISA based assay kits. If you are interested in our chemiluminescent immunoassay based kits development, please feel free to contact us for more details.

Published Data

1. Ultrasensitive Chemiluminescence Immunoassay for HE4 Detection Using Acridinium Ester-Labeled Antibody and GoldMag Nanoparticles

Fig.2 Schematic illustration of established sandwich-type GMP-CLIA method.Fig.2 The scheme of the GMP-CLIA method.2,4

By combining the AE chemiluminescence system with GoldMag nanoparticles (GMP), researchers presented a high-sensitivity and rapid chemiluminescence immunoassay (CLIA) for detecting human epididymis protein 4 (HE4). The AE labeling of antibodies process was systematically optimized by adjusting key factors, including the AE-to-antibody molar ratio, labeling duration, and the composition of the elution buffer and trigger solution. Under optimal conditions, AE labeling achieved an average efficiency of 1.92 ± 0.08 and an antibody utilization rate of 69.77 ± 1.19%, with no loss of antibody activity. The GMP-CLIA method successfully detected HE4 within a concentration range of 0.25-50 ng·mL−1 (10-2000 pM), with a lower detection limit of 0.084 ng·mL−1 (3.36 pM). This method demonstrated sensitivity comparable to commercial kits and was successfully applied to measure HE4 in 65 human serum samples, showing strong potential for clinical diagnosis.

2. Chemiluminescence Immunoassay for Diagnosing Mushroom Poisoning

Fig.3 Schematic illustration of the automated MB-based CLIA for quantitative detection of phallotoxins.Fig.3 Principle of MB-based CLIAs for automated detection of phallotoxins.3,4

In this study, researchers developed an ultrasensitive and automated chemiluminescence immunoassay (CLIA) using magnetic beads (MBs) for the early detection of mushroom poisoning. The method achieved limits of detection (LODs) for phallotoxins of 0.010 ng/ml in serum and 0.009 ng/ml in urine. Recovery rates ranged from 81.6% to 95.6%, with a coefficient of variation under 12.9%. The automated MB-based CLIA accurately identified Amanita phalloides samples, with results consistent with those from HPLC-MS/MS. The assay's exceptional sensitivity, reproducibility, and stability were attributed to the use of MBs as immunoaffinity carriers, chemiluminescence as a detection signal, and the complete automation of the process. This approach offers great potential for rapid clinical diagnosis of mushroom poisoning.

References

  1. Yasuda, Hiroshi, et al. "Unique biological activity and potential role of monomeric Laminin-γ2 as a novel biomarker for hepatocellular carcinoma: a review." International Journal of Molecular Sciences 20.1 (2019): 226. Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using only part of the original image.
  2. Ma, Ting, et al. "Preparation of an acridinium ester-labeled antibody and its application in goldmag nanoparticle-based, ultrasensitive chemiluminescence immunoassay for the detection of human epididymis protein 4." Micromachines 8.5 (2017): 149.
  3. Zhu, Jianyu, et al. "An automated and highly sensitive chemiluminescence immunoassay for diagnosing mushroom poisoning." Frontiers in Chemistry 9 (2021): 813219.
  4. Distributed under Open Access license CC BY 4.0, without modification.

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