Creative Biolabs has been devoted for the development of protein microarray-based kits, in which large numbers of proteins can be tracked in parallel. We also offer other ELISA kits for specific detection of disease-related proteins. Our professional team is optimized to help you with high-quality and cost-effective testing service to make your project a success.

How does it work?

Protein microarray is a high-throughput method for tracking protein interactions and activities, determining their function, and determining function on a large scale. The chip consists of a carrier surface such as a glass slide, nitrocellulose membrane, bead or microtiter plate, to which the capture protein array is bound. Probe molecules that are usually labeled with fluorescent dyes are added to the array. Any reaction between the probe and the immobilized protein emits a fluorescent signal and is read by a laser scanner. Protein microarrays are fast, automated, economical and highly sensitive, consuming small quantities of samples and reagents. The high-throughput technology behind protein microarrays is relatively easy to develop because it is based on technology developed for DNA microarrays, which have become the most widely used microarray. Protein array detection methods must provide a high signal and low background:

  • Fluorescence labeling. The most common and widely used method for detection for its highly sensitive, safe and compatible with readily available microarray laser scanners
  • Affinity tags
  • Photochemical tags
  • Radioisotope tags
  • Label free detection, such as carbon nanotubes, carbon nanowire sensors (where detection occurs via changes in conductance), surface plasmon resonance (SPR), and microelectromechanical system (MEMS) cantilevers

Fig.1 Antigen detection strategy in protein microarray technology.Fig.1 Workflow and antigen detection scheme in protein microarray technology.1,4

Protein Microarray Based Kits for IVD Development

Creative Biolabs has been devoted for the development of protein microarray-based kits, in which large numbers of proteins can be tracked in parallel. In vitro diagnostics includes detection of antigens and antibodies in blood samples; serum analysis to discover new disease biomarkers; monitoring of disease states and treatment responses in individualized diseases; monitoring of the environment and food. Digital bioassay is an example of diagnostics using protein microarrays. In this technique, a microwell array on a glass/polymer chip is seeded with magnetic beads (coated with fluorescently labeled antibodies), subjected to targeted antigens, and then characterized by microscopic counting of fluorescent wells. A cost-effective fabrication platform for the use of such microwell arrays (using OSTE polymers) have been demonstrated and biometric model systems have been successfully characterized.

Creative Biolabs is a world leader in the development of ELISA based kits and now offers protein microarray-based kits for specific detection of disease-related proteins. If you are interested in our ELISA kits development, please feel free to contact us for more details.

Published Data

1. Development and Validation of Protein Microarray for Simultaneous Detection of Inflammatory Mediators

Fig.2 The accuracy of microarray cytokine detection was calculated for serum.Fig.2 Microarray accuracy of cytokine detection.2

Researchers developed and validated an in-house microarray platform for simultaneous quantitative analysis of multiple protein biomarkers, including cytokines. The system's accuracy and precision were assessed according to FDA guidelines for pharmacokinetic assay validation, with results falling within acceptable limits for most biomarkers, except for very low-abundant cytokines like IL-10. Comparison with the "gold standard" ELISA format showed no significant differences in cytokine detection. The platform was not limited to the 16 cytokines demonstrated, offering potential for expansion to include additional biomarkers as needed. In conclusion, the researchers present a customized protein microarray system that utilizes well-established ELISA reagents, providing accurate, precise, and reproducible multiplexed biomarker quantification. The system offers flexibility and can be adapted beyond the capabilities of commercial microarray platforms.

2. Chemiluminescent Protein Microarray for Seroglycoid Fucosylation Index Determination

Fig.3 The schematic of the AFP antibody and LCA applications on the protein chip, and the protein microarray chip AFP/LCA antibody sandwich method.Fig.3 The schematic representation of the protein microarray method.3,4

In this study, researchers developed a chemiluminescent protein microarray that immobilized Alpha-fetoprotein (AFP)-specific antibodies and Lens culinaris agglutinin (LCA) onto aldehyde-treated glass slides. This method enabled the quantitative detection of AFP and/or fucosylated AFP (FucAFP) in biological samples and allowed the determination of the LCA-reactive fraction of AFP (AFP-L3)/ total AFP index (AFP-L3%). It addressed the challenge of lacking quantitative detection techniques for AFP and AFP-L3 in serum. The microarray assay presented in this study is highly sensitive, accurate, and efficient for measuring AFP, FucAFP, and AFP-L3%, providing a valuable tool for diagnostic applications.

References

  1. Beck, Sungjun, et al. "A protein microarray-based respiratory viral antigen testing platform for COVID-19 surveillance." Biomedicines 10.9 (2022): 2238.
  2. Selvarajah, Senthooran, et al. "Development and validation of protein microarray technology for simultaneous inflammatory mediator detection in human sera." Mediators of inflammation 2014.1 (2014): 820304. Distributed under Open Access license CC BY 4.0. The image was modified by extracting and using only part of the original image.
  3. Zhang, Aiying, et al. "A chemiluminescent protein microarray method for determining the seroglycoid fucosylation index." Scientific reports 6.1 (2016): 31132.
  4. Distributed under Open Access license CC BY 4.0, without modification.

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