Creative Biolabs is one of the leading custom antibody generation and development providers. We specialize in the generation of antibodies against a wide range of targets for R&D, diagnostic, and therapeutic applications. Currently, we have launched a series of in vitro diagnostic (IVD) antibody development services targeting numerous diagnostic biomarkers of human diseases. Here, we focus on alcohol dehydrogenase 5 (ADH5) as a marker of lymph cancer.
Alcohol Dehydrogenase 5 (ADH5)
The ADH5 gene encodes glutathione-dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which belongs to the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. Unlike other members of the ADH family with tissue-specific patterns of expression, ADH5 is ubiquitously expressed in both embryonic and adult tissues, suggesting a housekeeping function of this protein. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.
Fig.1 Structure of the ADH5 protein.1
ADH5 Marker of Lymph Cancer
ADH5, a well-conserved enzyme from bacteria to human, is involved in the metabolism of alcohols and aldehydes in mammalian cells. Gene expression analyses identify a constant reduction of ADH5 levels throughout neuronal development. Overexpression of ADH5 reduces both development and adult neuronal differentiation of mouse neurons. This effect depends on the catalytic activity of ADH5 and involves ADH5-mediated denaturization of histone deacetylase 2 (HDAC2). What's more, ADH5 has been found in several cancer types. The ADH5 stain may be helpful as a surrogate molecular marker for the classification of lymph cancers.
IVD antibodies are extensively used in immunodiagnostic tools for disease screening and therapeutic monitoring. Through our role as a leading antibody service provider, Creative Biolabs is well-positioned to develop high-quality ADH5-specific antibodies. Besides antibody generation, Creative Biolabs also offers diagnostic immunoassay development services, including feasibility analysis, assay design, assay protocol establishment, assay optimization, and kit production.
Creative Biolabs has successfully completed a number of IVD antibody generation and development projects for clients across the globe. If you are interested in our services, please contact us to discuss your project needs.
Reference
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