Creative Biolabs is the world leading service provider for the discovery of in vitro diagnostics (IVD) antibodies. Based on our years of experience and advanced platform, we are confident in offering the best and most suitable IVD assays service against various markers for cholangiocarcinoma.
miR-200b
MiR-200b belongs to the miR-200 family, which is composed of miR-200a, miR-200b, miR-200c, miR-141 and miR-429. These five members are located in two clusters, of which miR-200a/b/429 cluster is on chromosome 1p36 and the miR-200c/141 cluster is on chromosomal 12p13. miR-200 family plays a pivotal role in tumorigenesis. It has been observed that miR-200 family is downregulated in many carcinomas, such as lung, ovarian, breast cancer. Emerging studies have shown that the miR-200 family functions as tumor suppressors by regulating EMT (epithelial-mesenchymal transition), which is essential for tumor invasion and metastasis. Notably, miR-200b is the fundamental regulator in EMT beyond the other members of miR-200 family.
Regulation of miR-200 Family
The role of EMT in the progression of cancer is associated with miR-200 family by targeting ZEB1/2, which represses E-cadherin expression. It was found that overexpression of miR-200 prevents TGF-β induced ECM, in contrast, inhibition of miR-200 is sufficient to induce EMT. There has been a strong relation between ZEB1/2 and TGF-β and it was suggested that ZEB1/2 is crucial for TGF-β-induced EMT. The miR-200 family and ZEB1/2 are associated in a double-negative feedback loop, which can regulate EMT in tumorigenesis. In one side, miR-200 family target ZEB1/2 and suppress it to promote epithelial differentiation. On the other side, downregulation of ZEB1 can enhance miR-200. And this loop has a great effect on the stabilization of cellular differentiation.
Fig 1. The TGF-β/ZEB/miR-200 regulatory network. (Zaravinos, A., 2015)
miR-200b as a Diagnostic Marker for Cholangiocarcinoma
Several studies have shown that miR-200b is downregulated in some types of cancers including breast cancer, and it was suggested as a prognostic factor in breast cancer. Similarly, in Cholangiocarcinoma (CCA), it was found that four miR-200 members, including miR-200a/b/c429 are decreased. Furthermore, upregulated miR-200b/c prevents the migration and invasion of CCA cells. The mechanism that miR-200b/c influences the CCA tumorigenesis is involved the tumor-initiating capacity, sphere formation and drug resistance by directly targeting rho-kinase 2 and SUZ12. Hence, miR-200b also is an important prognostic biomarker for CCA.
With years of experience in IVD technology and assays, Creative Biolabs now provides the unique protocol to meet our clients' IVD demand for miR-200b. If you are interested in our service, please do not hesitate to contact us for more details.
Reference
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