Creative Biolabs provides in vivo pharmacodynamics studies in a validated, well-established animal model of carrageenan footpad edema, which is useful in the rapid evaluation of a test compound's anti-inflammatory activity. According to your unique requirements, our scientists will partner with you to assist you from initial study design to final reporting and analysis of your data to provide you with reliable, reproducible results.
Inflammation Models Induced by Carrageenan
The acute inflammatory response is well-known to be characterized by increased vascular permeability, increased blood flow, as well as infiltration of neutrophils and macrophages. Moreover, an edema is often formed due to exudation of fluid and plasma proteins and accumulation of leukocytes at the inflammatory site. To date, several experimental models of paw edema have been described by investigators. As one of the best characterized acute inflammatory model, carrageenan-induced mouse/rat model has been increasingly used to test new anti-inflammatory drugs as well as to study the mechanisms involved in inflammation.
Fig.1 Change in paw thickness (cm) after injected with carrageenan. (Sarika et al. 2012)1, 2
Induction of Carrageenan-Induced Paw Edema
Inflammation models induced by carrageenan are frequently used acute inflammation models mainly because they are well-researched and they exhibit a high degree of reproducibility. Carrageenan is a strong chemical that functions in stimulating the release of inflammatory and proinflammatory mediators, including bradykinin, histamine, tachykinins, reactive oxygen, and nitrogen species. Typical signs of inflammation include edema, hyperalgesia, and erythema, which develop immediately following the treatment of carrageenan.
Generally, animals are injected with a certain concentration (usually 1%) of the irritant substance carrageenan in one hind footpad, usually, half an hour or an hour after they are treated with the test compound while the other footpad is injected with saline as a control. The assessment of paw volume/thickness was performed at specified time points. Creative Biolabs provides other optional assessments including but not limited to:
Creative Biolabs also offers other rodent inflammatory & immunological disease models listed as follows that you may be interested in:
Creative Biolabs has high-end clinical technology platforms combined with experienced research and scientific teams that can support a wide range of services while ensuring consistent high-quality results and rapid turnaround time. Please feel free to contact us if you need more information or a formal quote.
References
For Research Use Only.
