Creative Biolabs has excellent experts good at comprehensive drug discovery services with years of experience. We can offer tailored digital imaging services using confocal laser scanning microscopy with the advanced functional analysis platform.
Confocal laser scanning microscopy (CLSM) is an optical imaging technique which is designed to improve comparison of a micrograph through augmenting a spatial pinhole to avoid out-of-focus light, of course, as well as to increase optical resolution. Combined with photomultipliers had high quantum potency in the near-ultraviolet, visible and near-infrared spectral areas, these microscopes enable to examine fluorescence emission arranging from 400 to 750 nanometers. CLSM allows reconstruction of three-dimensional structures from the procured images through gathering a series of images at different depths that usually named as optical sectioning. This technique is popularly applied to scientific or industrial communities, as well as semiconductor examination, life sciences, and materials science.
Figure 1. Confocal microscope configuration and information flow schematic diagram. (Claxton 2006)
Advantages of Confocal Laser Scanning Microscopy
The main advantage of CLSM is the capacity to consecutively bring in thin (0.5 to 1.5 micrometer) optical scarves by using fluorescent specimens which have a thickness ranging up to 50 micrometers or more. What's more, the non-invasive confocal optical sectioning dispels artifacts that exist during physical sectioning of tissue specimens which enables CLSM examining either living or fixed specimens under diverse states with intensive clarity. Finally, confocal scanning microscopy is capable of adjusting magnification electronically via changing the region scanned by the laser, without varying objectives.
Applications of Confocal Laser Scanning Microscopy
Generally, CLSM is applied to the clinical diagnosis of eye diseases and is specifically used for qualitative analysis, imaging, and quantification of endothelial cells of the cornea. For drug discovery process, it can be used for discriminating and localizing the existence of cell elements in the corneal stroma such as keratomycosis, allowing fast diagnosis and thereby inchoate institution of definitive therapy. CLSM plays a crucial role in materials analysis from fluorescence microscopy, optical sectioning as well as imaging perspectives. This method eliminates a number of disadvantages of light microscopy, for instance, a resolution blocked by the wavelength of light.
Creative Biolabs has focused on digital imaging studies for years, with the professional scientists hammering at drug discovery, our team is confident in providing incomparable digital imaging services using confocal laser scanning microscopy to meet every client's scientific research or clinical demands.
Creative Biolabs also provides other various drug discovery services. For more detailed information, please feel free to contact us or directly sent us an inquiry.
References
For Research Use Only.
