Creative Biolabs has built up a unique and comprehensive drug discovery service platform. Our team can provide customized digital imaging services using electron microscopy to meet every client's requirements.
Electron microscopy (EM) is a technique by using a variety of methods to acquire high-resolution images, either for biological or non-biological specimens. Generally, EM is applied to biomedical studies to check the particular structure of organelles, cells, tissues, and macromolecular complexes. Many techniques combined with EM are used for researching several particular questions, such as immuno-labeling, thin sectioning and negative staining. Among the numerous types of electron microscope, the scanning EM (SEM) and the transmission EM (TEM) are two types widely used. Besides, there are two new approaches of TEM: three-dimensional transmission electron microscopy (3D-TEM) and cryogenic transmission electron microscopy (cryo-TEM), which provide a new sight into the digital imaging research.
Figure 1. In situ electrochemical liquid cell transmission electron microscopy (TEM). (Unocic et al. 2014)
Transmission Electron Microscopy (TEM)
TEM is similar to a normal light microscope, and it can be applied to diverse studies, for instance, the structure of protein molecules, the interior of cells, the organization of molecules in viruses and cytoskeletal filaments, and the positioning of protein molecules in cell membranes. At present, TEM images are commonly recorded on photographic film with the silver halide emulsions. In addition, Cryo-TEM is useful in the research of several systems, for example, polymer and polymer-surfactant solutions, surfactant, biological and biomedical systems. Besides, 3D-TEM is a powerful tool in the study of single samples of inorganic solids several hundred nanometers in size.
Scanning Electron Microscopy (SEM)
The SEM is a technique used to generate images of a specimen through scanning it with a focused beam of electrons. SEM is able to offer diverse information on crystalline structure, chemical element, and surface topography. Moreover, it can accomplish resolution better than 1 nanometer. The majority of the sample surface is in focus synchronously whatever the surface roughness because the SEM is benefited from a large depth of field. The most usual SEM mode is an exploration of secondary electrons emitted by atoms activates by the electron beam. An image evidencing the topography of the surface enables to be generated through scanning the sample and gathering the secondary electrons which are emitted using a special detector.
Creative Biolabs has focused on drug discovery for years; our scientists are confident in performing perfect digital imaging services using electron microscopy. Creative Biolabs also provides other various drug discovery services. For more detailed information, please feel free to contact us or directly sent us an inquiry.
References
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