Antibody Reformatting and Production
As an experienced antibody solution provider, Creative Biolabs has emerged to be an excellent CRO that enables cutting-edge technologies and satisfied services. To support therapeutic antibody discovery, we have developed antibody reformatting and production service to facilitate your early discovery stage.
Antibody Reformatting Solutions
Antibody V regions (VH and VL) from a specific antibody can be reformatted into a variety of different fragments including Fab, F(ab)’2, scFv, VHH, minibodies, and also bispecific antibodies combined with a second specificity. Various antibody formats are optimized for tumor or tissue penetration, or expression in production systems. Here are our current available antibody reformatting choices.
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☆ Fab is monovalent and is approximately only a third of the size of a whole IgG;
☆ Fab has good characterized penetration properties into tissues and faster clearance from circulation;
☆ Fab can benefit from the absence of FcR receptor mediated effector functions;
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Can be easily expressed in non-mammalian expression systems.
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☆ Retain the bivalent binding of an IgG;
☆ Without Fc portion which mediates the effector functions via it’s interaction with FcR receptors.
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☆ Engineered by linking the variable domains of heavy chain and light chain into a single molecule;
☆ scFv is only approximately a fifth of the size of a whole IgG;
☆ Routinely used for phage display;
☆ Linked to a wide range of fusion proteins for specific targeting.
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☆ VHH only consists of the variable domain of the heavy chain;
☆ It’s only approximately a tenth of the size of a whole IgG;
☆ Highly stable and amenable to fuse with other proteins;
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Can be linked to Fc domains to be engineered as a heavy chain only antibodies.
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☆ Consist of two different binding domains which can target separate antigens;
☆ Critically useful when directing an effector cell to a target cell more effectively;
☆ Can be made in various formats;
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Can be designed to customize your requirements as per specific application.
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Our capabilities are not limited to above choices. At Creative Biolabs, we can help our clients to design and produce antibody fragments into virtually any formats via our antibody engineering program. We are dedicated to offering the best solutions which are tailored for your specific projects to ensure meeting the desired objectives. Our experienced team will collaborate with our clients from project initiating to results in the minimum amount of time. We aim to be your professional and attentive partner.
Creative Biolabs is committed to engineering antibodies into various formats, and also offering production services. Once produced, we characterize the antibodies with an extensive suite of functional assays such as ELISA, affinity determination, or other appropriate biological assays. High quality results will be delivered within timeline and budget requirements. To get more information, please contact us for a quote or to schedule a teleconference.
For Research Use Only | Not For Clinical Use
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