Aqueous Solubility Analysis Service

Unlocking Formulation Potential: Explore Solubility Solutions with Creative Biolabs

Creative Biolabs provides specialized in vitro absorption, distribution, metabolism, and excretion (ADME) services uniquely designed to support cancer immunotherapy research endeavors.

Aqueous solubility is a critical parameter in drug development, influencing drug absorption, bioavailability, and ultimately, therapeutic efficacy. Poor solubility may lead to suboptimal treatment outcomes. Therefore, accurate assessment of aqueous solubility is essential for informing formulation strategies and optimizing drug candidates for clinical use. Creative Biolabs specializes in providing high-quality aqueous solubility analysis services, leveraging advanced methodologies and cutting-edge technologies. Our expert team ensures precise and reliable measurements of compound solubility, supporting informed decision-making in drug development.

We employ a physiochemical assay to measure solubility. Using the HPLC-UV/VIS detection method, we analyze the peak area response to accurately determine the solubility of compounds. This precise approach ensures reliable data for optimizing drug formulations.

Here's an in-depth look at the methodologies and processes we employ:

• Shake Flask Method
  • Standard Shake Flask Method: This traditional method involves equilibrating the compound in an aqueous solution under controlled conditions, followed by filtration and analysis. It's a straightforward and reliable approach for routine solubility assessments.
  • Procedure: The compound is dissolved in a solvent, agitated for a specified period, and then filtered. The filtrate is analyzed using HPLC-UV/VIS to measure the peak area response, providing accurate solubility data.
• High Throughput Screening (HTS) Assays
  • Automated Liquid Handling Systems: Utilizing advanced automated systems, we conduct high-throughput screening to rapidly evaluate the solubility of extensive compound libraries. This method is ideal for early-stage drug discovery, allowing for the efficient identification of promising candidates.
  • Procedure: Compounds are dispensed into microtiter plates, mixed with the solvent, and incubated. The solubility is then assessed using HPLC-UV/VIS, enabling quick and accurate solubility profiling.
• Thermodynamic Solubility Determination
  • Thermodynamic Solubility Measurements: We employ techniques such as UV-Vis spectroscopy and HPLC to determine the intrinsic solubility of compounds. This method focuses on understanding the solubility at equilibrium, providing deeper insights into the compound's solubility characteristics.
  • Procedure: We dissolved the compound in an aqueous buffer and allowed it to reach equilibrium. Samples are then filtered and analyzed using HPLC-UV/VIS to measure the peak area response, providing data on the compound's thermodynamic solubility.
• HPLC-UV/VIS Detection Method
  • HPLC with UV/VIS Detection: This method involves separating the compound from impurities and measuring its concentration in the solution using UV/VIS detection. The peak area response from the HPLC-UV/VIS analysis is used to quantify the solubility of the compound accurately.
  • Procedure: After preparing the sample, it is injected into the HPLC system. The separation occurs in the column, and the UV/VIS detector measures the peak area, correlating to the concentration of the compound in the solution.

Conditions We Employ:

Fig.1 Conditions available for aqueous solubility.

Applications

• Formulation Development: Assess the aqueous solubility of drug candidates to guide formulation development and enhance drug delivery strategies.
• Lead Optimization: Screen compound libraries to identify lead candidates with favorable aqueous solubility profiles for further development.
• Bioavailability Enhancement: Evaluate the impact of formulation modifications on compound solubility to improve drug bioavailability and efficacy.

Advantages

• Our assays provide precise and reproducible measurements of compound solubility, ensuring confidence in the data obtained.
• We offer tailored assay designs and flexible service options to meet the specific needs of your research projects, ensuring optimal outcomes and data relevance.

Creative Biolabs offers a full panel of aqueous solubility services for drug products. We provide clients with full reports including the raw data, the methods that were used as well as the results of the analysis to help in decision-making. With the use of these up-to-date approaches and advanced technologies, Creative Biolabs guarantees accurate solubility detection results that facilitate your drug development generations with reliable data. Get in touch to discover your solubility solutions and fast-track your way through the drug discovery pipeline.

For Research Use Only | Not For Clinical Use