Bispecific Antibody Engineering
Creative Biolabs is a leading supplier of antibody engineering and optimization for immunotherapy and tumor research. Our antibody engineering team provides an independent and comprehensive service portfolio, ranging from bispecific antibody production to optimized multipurpose bispecific antibodies, particularly in immune therapeutics.
The specific items including antibody affinity maturation, antibody expressing and stability optimization, scFv / Fab formats design and construction, antibody humanization and other effector function optimization services.
Bispecific Antibody Engineering Workflow
Bispecific Antibody Design
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Choosing a format that is compatible with the target MoA is critical
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Computer simulation for 3D structural modeling
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After extensive computer simulation testing, make reasonable modifications in the BsAb interface, such as introducing appropriate mutations, charged residues and hydrophobicity - polarity exchange
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Predicted three-dimensional structural model after computer simulation testing of many potential mutations
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Initial testing using software such as the Designer program
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Using the MM-GBSA method to assess the binding free energy, correct pairing, and mismatch of the mutant format
Key Advantages
✔ Our humanization strategy minimizes the risk of loss of affinity and functional activity caused by traditional CDR transplantation methods.
✔ We use randomization and mutagenesis of selected CDRs to provide affinity maturation services to optimize antibody affinity and cross-reactivity with orthologous target antigens.
✔ Our Fc engineering uses site-specific mutations and glycosylation modifications to enhance the stability of antibodies in vivo and ADCC / CDC potency.
✔ We have extensive experience in the production of thousands of engineered antibodies and the fastest delivery time.
For more details, please feel free to contact us for project quotations and more detailed information.
For Research Use Only | Not For Clinical Use
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