BJ Cell-based Cytotoxicity Safety Screening Service
In Vitro Cytotoxicity Screening Testing
The development of new pharmaceutical compounds needs rigorous toxicity testing to ensure their safety and efficacy before they can be deemed suitable for human use. Toxicity testing is a critical step in the drug development process, serving to identify any adverse effects that a compound may have on biological systems. One key aspect of this process is cytotoxicity screening, which evaluates the potential harmful effects of drug candidates on cellular health and function. By evaluating the potentially toxic effects of new compounds on cultured cells, researchers can identify and eliminate harmful candidates before they proceed to animal testing or clinical trials. Creative Biolabs is dedicated to offering high-throughput, cell-based cytotoxicity safety screening services, providing a reliable and efficient platform for assessing the safety profile of drug candidates. These advanced services enable the rapid and efficient evaluation of drug candidate safety, ultimately contributing to the development of safer therapeutics.
BJ cell-based Cytotoxicity Safety Screening Service
Creative Biolabs offers state-of-the-art, quantitative high-throughput screening services designed specifically to profile the cytotoxicity of novel compounds on BJ cells. Utilizing a homogeneous, luminescent cell viability assay, this service provides an accurate, sensitive measure of cytotoxic effects, crucial for determining the safety profile of drug candidates. This advanced screening technique allows for the simultaneous testing of a large number of compounds, vastly improving efficiency and throughput. By leveraging this high-throughput approach, researchers can rapidly accumulate reliable data, accelerating the decision-making process and ultimately expediting the journey from bench to bedside.
Brief Overview of Our Method
The process begins with seeding BJ cells into a microplate, followed by the compound treatment. After allowing an incubation period for cellular response, a luminescent reagent is added. The reagent lyses the cells and stabilizes the released ATP, which then produces a luminescent signal through an enzymatic reaction. The intensity of the luminescent signal is measured and directly correlates with cell viability, thereby providing a quantitative measure of cytotoxicity.
Frequently Asked Questions
1. Why use BJ cells for cytotoxicity safety screening?
BJ cells, which are human foreskin fibroblasts, represent a relevant and reliable model for cytotoxicity testing due to their human origin and fibroblast nature. They are particularly useful for evaluating the cytotoxic effects of compounds on human connective tissue cells, offering insights into potential adverse effects on human dermal and sub-dermal layers. Additionally, BJ cells are relatively easy to culture and maintain, which facilitates reproducible and consistent results in cytotoxicity assays.
2. Which assay do you use in cytotoxicity safety screening?
We use a luminescent cell viability assay that measures ATP levels, which serve as an indicator of metabolically active (viable) cells, with luminescence being proportional to the amount of ATP present. We also offer a variety of other cytotoxicity assays. Please contact us to customize your testing requirements.
For Research Use Only | Not For Clinical Use
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