Caco-2-based Efflux Transporter Substrate Assessment Service
Creative Biolabs provides a reliable and efficient method to evaluate the substrate potential of compounds for efflux transporters in the human intestine. Caco-2 cells are commonly used as a model to assess drug permeability and efflux
transporter activity due to their ability to mimic human intestinal epithelial cells.
Efflux Transporter
Efflux transporters are membrane proteins that actively transport various molecules out of cells. These transporters play a key role in protecting cells from toxic substances and keeping cellular homeostasis. A substrate is a molecule that can be recognized
and transported by an efflux transporter. The specificity of efflux transporters can vary widely, with some transporters able to recognize a broad range of substrates while others may have a more narrow substrate specificity.
Caco-2-based Efflux Transporter Substrate Assessment Service
The Caco-2 efflux transporter substrate panel is a group of compounds that have been tested to be able to transport out of cells by efflux transporters in the Caco-2 cell line. Testing these compounds in Caco-2 models can help you assess the potential
for efflux transporter-mediated drug interactions and inform drug development strategies.
Process of Our Service
The process of our Caco-2-based efflux transporter substrate assessment service includes sample preparation, assay setup, measurement efflux transport, analysis of data, and reporting of results.
1
Sample preparation
Preparing the samples for assessment is the first step. The cells are then treated with the substrate of interest.
2
Assay setup
Samples are set up in an appropriate assay format.
3
Efflux transport measurement
The efflux transport of the substrate is then measured using appropriate analytical techniques.
4
Data analysis
The data collected from the efflux transport measurement is then analyzed to determine the extent of substrate transport across the Caco-2 cell monolayer.
5
Reporting
A detailed report is then generated summarizing the findings of the efflux transporter substrate assessment.
Assay Information
Assay Sub Type
Cell Based
Detection Method
HPLC-MS/MS
Measured Response
Peak Area Response
Key Features
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Assessment of efflux transporter activity
We use Caco-2 cells to evaluate the efflux transporter activity of compounds by measuring their transport across a monolayer of cells.
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Quantitative analysis
We provide quantitative data on the permeability of compounds and their interaction with efflux transporters.
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Customized study design
Our technicians work closely with our customers to design studies that meet their specific needs and goals. Whether you are interested in screening a large number of compounds or studying the mechanism
of efflux transporter interactions, we tailor our services to your requirements.
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Expert guidance
Our team of scientists has extensive experience in Caco-2-based assays and efflux transporter assessment. Our scientists provide expert guidance and advice to help you interpret the results of your project.
Frequently Asked Question
A1: At what stage is the Caco-2-based Efflux Transporter Substrate Assessment performed?
Q1: The Caco-2-based Efflux Transporter Substrate Assessment is typically performed during the early stages of drug development, when evaluating the potential for a compound to be a substrate for efflux transporters such as P-glycoprotein (P-gp). If you
have any questions about this, you can get in touch with our technicians directly for more details.
Contact Us
Overall, our Caco-2-based efflux transporter substrate assessment service offers a comprehensive and reliable approach to evaluating the substrate potential of compounds for efflux transporters. If you have any efflux transporter substrate assessment
needs, please do not hesitate to contact us and we will fully assist you with your research and immunotherapy development efforts.
For Research Use Only | Not For Clinical Use
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