Creative Biolabs uses its unique calcium ion homeostasis pathway analysis technology to thoroughly investigate the impact of drugs on calcium ion balance in cardiomyocytes, enabling the assessment of their potential cardiotoxic effects. We can assist clients in identifying and addressing cardiotoxicity risks early in the drug development process, thereby facilitating the emergence of safe and effective cancer therapies.

Introduction

Calcium ion homeostasis plays a crucial role in the cardiotoxicity induced by anticancer drugs, and research in this area has progressed rapidly in recent years. Anticancer drugs, particularly certain chemotherapy agents and targeted therapies are linked to cardiotoxicity. These drugs disrupt calcium ion balance in cardiomyocytes through various mechanisms, ultimately resulting in impaired cardiac function.

In recent years, Creative Biolabs has offered cell biology experiments and molecular biology analyses related to calcium ion homeostasis to investigate the effects of drugs on cardiac cells, such as cardiomyocytes and cardiac endothelial cells. Moreover, we assist clients in developing and refining in vitro and in vivo models to accurately assess the cardiotoxicity resulting from calcium ion homeostasis imbalances caused by anticancer drugs. Through comprehensive data analysis, our scientists ultimately provide risk assessment services for drug-induced cardiotoxicity and identify key calcium ion steady-state pathways and associated pathological mechanisms.

Fig.1 Calcium Homeostasis.^1,3

Services

Creative Biolabs provides multidisciplinary research services that delve into the impact of calcium ion homeostasis disruption on cardiac function. By integrating cell biology, electrophysiology, and pharmacology, we conduct comprehensive and detailed calcium ion steady-state pathway assessments. Our studies leverage cutting-edge imaging and sensor technologies to achieve highly sensitive monitoring of calcium ion dynamics.

• Calcium Channel Function Evaluation: Creative Biolabs employs electrophysiological techniques to assess the activity and functionality of calcium channels, examining the impact of anticancer drugs on cardiac cell calcium channels.
• Intracellular Calcium Ion Mobilization Measurement: Utilizing fluorescent dyes or calcium sensor technologies, we can monitor real-time changes in calcium ion concentration within myocardial cells or cardiac tissues.
• Cell Apoptosis and Necrosis Analysis: Our labs are equipped to evaluate the effects of anticancer drugs on the survival of cardiac cells, including pathways induced by calcium overload leading to apoptosis and necrosis.
• Animal Model Studies: Our team can assess the cardiotoxicity of anticancer drugs in animal models, such as mice or rats, integrating physiological parameter monitoring with pathological analyses.
• Signal Pathway Analysis: We use methods like western blotting and qPCR to measure changes in the expression and activity of calcium signaling pathways and related downstream effectors.

Fig.2 Intracellular Ca²⁺ Mobilization Analysis.^2,3

Features

Myocardial fibrosis is often associated with various cardiac pathological conditions, including hypertension, cardiac ischemia, myocarditis, and heart toxicity induced by drugs or chemicals. Currently, gaining a deeper understanding of the molecular mechanisms underlying myocardial fibrosis, particularly the changes in relevant pathways, is crucial for the prevention, early diagnosis, and treatment of these diseases. Creative Biolabs employs the latest bioinformatics tools to analyze gene expression data provided by clients, identifying key signaling pathways related to myocardial fibrosis. These pathways may include TGF-β, Wnt, NF-κB, and PI3K/Akt, among others.

• Specialized Research:

Focused and in-depth exploration of the mechanisms by which calcium ions contribute to cardiotoxicity. Using both cellular and animal models to facilitate comprehensive monitoring of calcium ion steady-state pathway.

• Multifaceted Analysis:

Integrating molecular biology, cell physiology, and pharmacokinetics to deliver a thorough analysis of calcium ion steady-state pathway. This includes variations in intracellular calcium levels, as well as the activation and inhibition of calcium signaling pathways.

• Top-class Technology:

Employing advanced techniques such as high-throughput screening, fluorescence imaging, and mass spectrometry for precise measurement of calcium fluctuations induced by anticancer drugs.

• Customized Services:

Customizing experimental designs to align with client requirements, which involves evaluating cardiotoxicity for particular medications and screening drugs that focus on the regulation of calcium ion steady-state pathways.

• Comprehensive Data Interpretation:

Delivering detailed analysis and interpretation of data, encompassing the mechanisms of calcium ion steady-state pathway, potential assessments of cardiotoxicity, and their relevance in preclinical research.

Creative Biolabs specialized CRO analysis services focused on calcium ion steady-state pathways related to cardiotoxicity in the development of novel anti-cancer drugs. Our services encompass a range of offerings, including precise monitoring of calcium ion flux, assessments of cell viability, evaluations of cardiomyocyte functionality, and full analyses of cardiotoxicity mechanisms. We invite you to contact us for consultation, as we work together to advance the development and safety of anti-cancer therapies!

References

1. Zheng, Shanliang, et al. "Calcium homeostasis and cancer: insights from endoplasmic reticulum-centered organelle communications." Trends in Cell Biology 33.4 (2023): 312-323.
2. Saha, Sankhanil, Harini Krishnan, and Padinjat Raghu. "IMPA1 dependent regulation of phosphatidylinositol 4, 5-bisphosphate and calcium signaling by lithium." Life Science Alliance 7.2 (2024).
3. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use