Cancer Microbiome Profiling Service
Creative Biolabs is proud to offer cutting-edge Cancer Microbiome Profiling Service, a comprehensive solution for the analysis and characterization of the cancer microbiome. This cutting-edge service enables in-depth analysis of the cancer-associated microbiome, providing valuable insights into the disease mechanisms and potential therapeutic strategies.
Methods for Cancer Microbiome Studies
Creative Biolabs employs a variety of advanced molecular techniques to characterize the cancer microbiome. These methods include:
16S rRNA Gene Sequencing
This widely used technique allows for the identification and profiling of bacterial communities based on the highly conserved 16S rRNA gene. By sequencing specific regions of this gene, our experts can accurately determine the taxonomic composition of the microbiota present in cancer samples.
Metagenomic Shotgun Sequencing
By sequencing the entire DNA content of the microbial community, metagenomic shotgun sequencing provides a comprehensive view of the cancer microbiome. This approach enables the identification of not only bacteria but also viruses, fungi, and other microorganisms, providing a more detailed understanding of the microbial ecosystem.
Metatranscriptomic Sequencing
This method involves sequencing the RNA transcripts within the microbiome, allowing the analysis of gene expression patterns. By examining the active genes and pathways within the cancer microbiome, we can gain insights into the functional potential and activity of the microbial community.
Analyses for Cancer Microbiome Studies
Creative Biolabs provides a diverse array of technological strategies to detect, identify, and pinpoint microorganisms such as bacteria and yeasts in both simple and complex samples derived from preclinical investigations or clinical trials.
Detection
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CFU counting
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qPCR coupled or not to PMA
Identification
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Mass spectrometry
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qPCR/dPCR
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16S rRNA gene sequencing
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Shotgun sequencing
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Phylopeptidomics
Localization
Workflow for Cancer Microbiome Analysis
Creative Biolabs follows a standardized workflow to ensure accurate and reproducible analysis of the cancer microbiome. The key steps involved in our Cancer Microbiome Profiling Service include:
Sample Collection and Preservation
We provide detailed guidelines for the collection and preservation of cancer samples to maintain the integrity of the microbiome. Proper storage conditions and transportation protocols are crucial to obtain reliable results.
DNA/RNA Extraction
Our experienced technicians employ optimized protocols to extract high-quality DNA or RNA from the cancer samples. This step is critical for downstream sequencing and analysis.
Library Preparation
We perform library preparation according to the selected sequencing method (16S rRNA gene sequencing, metagenomic shotgun sequencing, or metatranscriptomic sequencing). This step involves amplification, fragmentation, and adapter ligation to generate sequencing-ready libraries.
Sequencing
High-throughput sequencing platforms are utilized to generate vast amounts of sequencing data. Our state-of-the-art facilities ensure accurate and efficient sequencing of the cancer microbiome.
After HT sequencing, our bioinformatics team utilizes sophisticated algorithms and computational tools to process the sequencing data. This includes data quality control, taxonomic profiling, functional annotation, and statistical analysis to unravel the complexity of the cancer microbiome.
Sample Types for Cancer Microbiome Analysis
The Cancer Microbiome Profiling Service offered by Creative Biolabs can analyze various types of cancer samples, including:
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Tumor Tissue: Profiling the microbiome within the tumor microenvironment can provide insights into the interaction between cancer cells and microorganisms. This analysis helps identify potential biomarkers and therapeutic targets.
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Fecal Samples: The gut microbiome has been extensively studied for its role in cancer development and response to treatment. Analysis of fecal samples allows for non-invasive monitoring of the cancer-associated microbiome and its potential impact on disease progression.
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Biofluid Samples: Analysis of biofluids, such as blood, urine, and feces, allows for non-invasive monitoring of the cancer microbiome and potential biomarkers.
Applications for Cancer Microbiome Analysis
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Cancer Biomarker Discovery:
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Therapeutic Targets
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Immunotherapy Optimization
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Microbiome-based Therapeutics
Please feel free to contact us for more about our Cancer Microbiome Profiling services.
For Research Use Only | Not For Clinical Use
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