Cardiac cell metabolism-based analysis services provided by Creative Biolabs are essential for our worldwide clients focusing on cardiovascular diseases, drug development, and cell biology. These services typically encompass a range of analytical and experimental techniques designed to understand the metabolic profiles of cardiac cells under various conditions.

Introduction

The heart is an organ that heavily relies on energy, primarily utilizing fatty acids and glucose for its energy production. Understanding the metabolic processes of cardiac cells is essential for comprehending their function and the health implications. Metabolic analysis can be used to evaluate the effects of drugs on cardiac cells, identify early biomarkers for diseases, and uncover potential mechanisms of cardiotoxicity. Nowadays, Creative Biolabs primarily offers several aspects of cardiac-related cellular metabolism analysis:

• Energy Metabolism Assessment: We evaluate energy metabolism pathways within cardiac cells, such as oxidative phosphorylation and glycolysis, and how these pathways are affected by various drug treatments.
• Metabolite Analysis: Our labs employ techniques like mass spectrometry (LC-MS and GC-MS) to analyze small-molecule metabolites in cardiac cells or relevant bodily fluids, identifying critical metabolites associated with cardiotoxicity.
• Cellular Respiration Analysis: Creative Biolabs utilizes instruments like the Seahorse XF Analyzer to monitor the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of cells in real-time, allowing us to assess their energy metabolism status.
• Metabolomics Research: Our team extensively applies metabolomics techniques to investigate metabolic changes under different cardiac diseases or drug effects, aiming to uncover potential biomarkers.

Fig.1 Metabolic Profile in Aging Hearts.^1,3

Services

Creative Biolabs focuses on researching cardiac toxicity and offers comprehensive cellular metabolic analysis solutions for its clients. With our advanced technology and specialized team, we can uncover the metabolic characteristics of cardiac cells under various physiological and pathological conditions through in-depth metabolomics studies.

Our services include cell culture, metabolic pathway analysis, metabolite screening, and characterization, as well as the development of related biomarkers. These offerings are designed to help clients understand the dynamic changes in cardiac metabolism and its implications for cardiovascular diseases. By integrating high-throughput analytical techniques with precise data interpretation, we ensure that we provide accurate and reliable research outcomes, facilitating the development of new drugs and optimizing treatment strategies.

Fig.2 Glutamate Metabolism Analysis in Heart Models.^2,3

Features

Creative Biolabs uses a wide array of technologies and analytical techniques to conduct well-mature studies of the metabolic processes in cardiac cells. This comprehensive approach empowers our clients to make significant advancements in areas like fundamental research, drug development, and preclinical studies. Whether you need specialized experimental designs, data analysis, or insights into metabolic mechanisms, we are committed to offering you expert guidance and customized solutions.

• Metabolic Flux Analysis: Assess the metabolic rates of cells through various pathways, including both aerobic and anaerobic respiration.
• Metabolite Detection: Utilize mass spectrometry or nuclear magnetic resonance (NMR) techniques to identify metabolites inside and outside cardiac cells, aiding in the evaluation of metabolic status.
• Oxygen Uptake and Carbon Dioxide Release Measurement: Analyze cellular respiration to understand how cells utilize oxygen and produce carbon dioxide.
• Energy Metabolism Assessment: Evaluate ATP production, energy storage, and the variations under different conditions.
• Gene Expression Analysis: Investigate the expression levels of metabolism-related genes through transcriptomics to explore mechanisms of metabolic regulation.
• Pharmaceutical Impact Studies: Assess the effects of specific drugs or treatments on the metabolism of cardiac cells, including their modulation of energy metabolic pathways.
• High-Throughput Screening: Leverage high-throughput techniques to identify compounds that can affect the metabolism of cardiac cells, in the quest for potential drug candidates.
• Data Analysis and Modeling: Provide metabolic modeling and data analysis services to enhance the understanding of complex metabolic networks and their dynamic changes.

Whether for basic research or preclinical trials, Creative Biolabs can tailor experimental protocols to meet the specific needs of our clients, advancing scientific research in the field of cardiotoxicity analysis & prevention. By choosing our CRO services for cardiac cell metabolic analysis, you will gain access to industry-leading technological support and professional services, embarking on a journey to explore new frontiers in cardiotoxicity studies. Feel free to contact us at any time to discuss your project needs. We look forward to supporting your cardiac cell metabolic research!

References

1. Xie, Saiyang, et al. "Metabolic landscape in cardiac aging: insights into molecular biology and therapeutic implications." Signal transduction and targeted therapy 8.1 (2023): 114.
2. Kutschka, Ilona, et al. "Activation of the integrated stress response rewires cardiac metabolism in Barth syndrome." Basic Research in Cardiology 118.1 (2023): 47.
3. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use