Luciferase Cell Line Products

A Luciferase Cell Line is a genetically engineered cell line designed to express the luciferase enzyme. This enzyme catalyzes a bioluminescent reaction, producing light in the presence of its substrate (commonly luciferin), and is widely used in research to monitor cellular activity. By tagging genes or proteins of interest with the luciferase marker, you can quantify biological processes, such as gene expression, protein interactions, and cell viability, through luminescence intensity. Luciferase cell lines are particularly valuable for high-throughput screening, real-time imaging, and in vitro and in vivo studies, offering a sensitive, non-invasive, and quantifiable method to analyze various cellular functions and responses.

Advantages of Luciferase Cell Lines

High Sensitivity

Highly sensitive, enabling detection of low-level biological activities or signals, making it ideal for studying weak gene expression or low-abundance proteins.

Real-Time Monitoring

Luminescence produced by luciferase can be monitored in real-time, allowing you to observe dynamic cellular processes continuously and non-invasively.

Quantitative Analysis

Luminescence intensity correlates with luciferase activity, providing a direct and quantifiable readout of gene expression, cellular response, or protein interactions.

Low Background Noise

Luciferase reactions have minimal background signal since mammalian cells lack endogenous luciferase, leading to clearer, more accurate results.

High Throughput Compatibility

The luciferase assay's simplicity and sensitivity make it compatible with high-throughput screening.

Broad Utility

Luciferase cell lines are versatile, suitable for various applications.

Non-Toxic and Non-Destructive

Luciferase expression and luminescence do not harm cells, enabling multiple time-point measurements on the same sample without disrupting cellular health.

Featured Products

Cat. NO	Product Name	Optical Reporter	Quantity
IOC-ZP014	CellOnco™ Human HepG2-Luc Reporter Tumor Cell Line	Luciferase	1x10^6 cells/vial
IOC-02P033	CellOnco™ Mouse CT26-Luc Tumor Cell Line	Luciferase	1x10^6 cells/vial
IOC-ZP003	CellOnco™ Mouse GL261 Luc Reporter Tumor Cell Line	Luciferase	1x10^6 cells/vial
IOC-ZP129	CellOnco™ Human NALM6-Luc Reporter Tumor Cell Line	Luciferase	1x10^6 cells/vial
IOC-ZP059	CellOnco™ Human U-251-Luc Reporter Tumor Cell Line	Luciferase	1x10^6 cells/vial
IOC-ZP002	CellOnco™ Human K562 Luc Reporter Tumor Cell Line	Luciferase	1x10^6 cells/vial

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Applications of Luciferase Cell Lines

Gene Expression Studies
Allowing researchers to analyze promoter activity, gene regulation, and transcriptional responses to stimuli.
Drug Screening and Development
Measure the impact of chemicals on target genes or pathways to enable high-throughput screening of possible therapeutic candidates.
Cell Viability and Cytotoxicity Assays
Assess the cytotoxicity of drugs or environmental factors, providing insights into cellular health and apoptotic pathways.
Signal Transduction Pathway Analysis
Monitor specific signaling pathways (e.g., NF-kB, CREB, or Wnt pathways), aiding in understanding how cells respond to various stimuli or drugs at the molecular level.
Protein-Protein Interactions
Study protein interactions within cells, providing insights into complex cellular processes and identifying therapeutic targets.

Workflow of Luciferase Cell Lines

Published Data

Fig.1 Assay readout. (Mosaad, et al., 2019) Fig.1 The figure above shows the assay readout of luciferase in a single cell culture.¹

Contact Us for Custom Luciferase Cell Lines

If you are interested in incorporating luciferase technology into your research or need a specific luciferase cell line tailored to your project requirements, we are here to help. As a trusted supplier of custom luciferase cell lines, we specialize in developing cell models optimized for sensitivity, stability, and efficiency to meet your unique experimental needs. For more information on our custom luciferase cell line offerings, or to discuss your project specifics, please contact us today. We look forward to supporting your research with high-quality, reliable luciferase cell lines designed to enhance your results.

Reference

Mosaad, E.O et al. "Constraints to counting bioluminescence producing cells by a commonly used transgene promoter and its implications for experimental design." Sci Rep 9, 11334 (2019). Distributed under Open Access license CC BY 4.0, without modification.