Overexpression Cell Line Products

An overexpression cell line is a genetically modified cell line designed to express a specific gene at significantly higher levels than normal. This is achieved by introducing a gene of interest into a suitable expression vector, typically containing a strong promoter for enhanced transcription. Overexpression cell lines are widely used in molecular biology and biotechnology to study the functions and characteristics of proteins, investigate gene regulatory mechanisms, and explore the consequences of increased gene activity. Researchers often utilize these cell lines to analyze cellular processes such as proliferation, differentiation, and response to drugs, making them valuable tools for drug discovery, disease modeling, and therapeutic development. By providing insights into the molecular underpinnings of various biological phenomena, overexpression cell lines play a crucial role in advancing our understanding of genetics and cellular biology.

Advantages of Overexpression Cell Lines

Enhanced Protein Production

Facilitating biochemical studies, structural biology research, and protein purification for various applications.

Functional Studies

Providing insights into signaling pathways, metabolic functions, and other essential cellular mechanisms.

Disease Modeling

Helping to elucidate disease mechanisms and identify potential therapeutic targets.

Pathway Analysis

Allowing for the identification of upstream regulators and downstream effects.

Drug Response Assessment

Evaluate how increased expression of target proteins affects cellular responses to drugs, including sensitivity or resistance, which is crucial in drug discovery and development.

Genetic Screening

Combined with high-throughput screening techniques to find substances that alter the overexpressed protein's activity, assisting in the development of novel medicinal treatments.

Featured Products

Cat. NO	Product Name	Cell Line	Overexpressed Gene
ITS-0624-HMM1	CellOnco™ HEK293T-Tg(Human A1 Receptor) Stable Cell Line	HEK293T	A1 Receptor
ITS-0624-HMM73	CellOnco™ HEK293T-Tg(Human CB1 Receptor) Stable Cell Line	HEK293T	CB1 Receptor
ITS-0822-HMM34	PharmaOnco™ CHO-K1/CD112 Stable Cell Line	CHO-K1	CD112
ITS-0822-HMM37	PharmaOnco™ CHO-K1/Mouse CD155 Stable Cell Line	CHO-K1	CD155

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Applications of Overexpression Cell Lines

Molecular Biology
Studying gene function and regulation, including the effects of gene overexpression on cellular pathways and mechanisms.
Cancer Research
Examining how tumor suppressor genes and oncogenes affect the development, spread, and response to treatment of cancer.
Stem Cell Biology
Exploring the effects of overexpressing transcription factors on stem cell pluripotency, self-renewal, and differentiation.
Drug Discovery and Development
Validating potential therapeutic targets by assessing the effects of drug candidates on cell lines with overexpressed target proteins.
Neuroscience
Studying the effects of gene overexpression on neuronal function, synaptic plasticity, and neurodegenerative diseases.

Workflow of Overexpression Cell Lines

Published Data

Fig.1 Overexpressed cell line. (Jeffery, et al., 2021) Fig.1 The figure above shows the generation protocol for an overexpressed cell line.¹

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Reference

Jeffery, Daniel et al. "CENP-A overexpression promotes distinct fates in human cells, depending on p53 status." Communications biology vol. 4,1 417. Distributed under Open Access license CC BY 4.0, without modification.