Cell-based ASBT Substrate Assessment Service
Cell-based ASBT substrate assessment service is a cutting-edge technology that enables you to quickly and accurately assess the substrate specificity of the human Apical Sodium-Dependent Bile Acid Transporter (ASBT). By utilizing cell-based assays, Creative Biolabs provides valuable insights into how ASBT interacts with various substrates, helping you to understand the underlying mechanisms of bile acid absorption and metabolism.
ASBT
ASBT, or apical sodium-dependent bile acid transporter, is a protein that plays a critical role in the uptake of bile acids in the intestine. The ASBT substrate refers to the specific molecules that can be transported by ASBT. Bile acids, such as taurocholic
acid and glycocholic acid, are examples of ASBT substrates. These substrates are taken up by ASBT in the intestine and then transported into the cells for further processing and metabolism. The ASBT substrate specificity allows for the efficient
absorption of bile acids, which is essential for the digestion and absorption of dietary fats.
Cell-based ASBT Substrate Assessment Service
Our service is primarily used in drug discovery and development to assess the potential of a compound to inhibit or enhance the activity of ASBT, which plays a crucial role in the absorption of bile acids in the intestines. The process starts with the
selection of appropriate cell lines that express ASBT, such as Caco-2 cells or HEK293 cells transfected with the ASBT gene. The above cells are cultured and maintained under specific conditions to ensure their viability and functionality. The substrate
of interest, which can be a drug candidate or a natural compound, is then introduced to the cells at a range of concentrations. The cells are incubated with the substrate for a predetermined period of time, allowing for the substrate to be taken up
by the ASBT transporters on the cell membrane. After the incubation period, cells are harvested and analyzed to measure substrate uptake by ASBT. This can be done using various techniques, such as liquid chromatography-mass spectrometry (LC-MS) or
fluorescence imaging.
Process
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ASBT substrate assessment
Cell Type We Used
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HEK293 cells
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Caco-2 cells
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MDCKII
Assay Information
Cell Type
Mentioned above
Assay Sub Type
Cell Based
Detection Method
HPLC-MS/MS
Measured Response
Peak Area Response
Features of Our Service
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High-throughput screening
Our service allows for the rapid screening of large numbers of potential substrates for the ASBT transporter using cell-based assays.
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Customizable assay design
Our assay can be tailored to meet the specific needs of the research project, including different cell lines, transporter expression levels, and assay conditions.
Our service provides real-time data on substrate uptake by the ASBT transporter, allowing for the immediate assessment of substrate transport properties.
The use of cell-based assays ensures the accuracy and reproducibility of the substrate assessment results.
Our service includes consultation with experienced scientists who can provide guidance on assay design and interpretation of results.
Our service provides fast results, allowing you to efficiently evaluate the potential of substrate compounds for ASBT transport.
Our service includes data analysis and reporting to help you interpret the results of the substrate assessment.
Contact Us
If you are interested in learning more about our cell-based ASBT substrate assessment services, please do not hesitate to contact us. Our team of experts is ready to assist you with any inquiries you may have and provide
you with more information on how we can help meet your specific needs. Whether you are looking to evaluate the efficacy of a new substrate or need assistance with optimizing your current ASBT assays, we are here to support you every step of the way.
For Research Use Only | Not For Clinical Use
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