Cell-based Mitochondrial Toxicity Analysis Service

Explore Mitochondrial Dynamics with Creative Biolabs' Toxicity Analysis

Evaluating the impact of compounds on cellular structures and functions, such as mitochondria, is crucial for identifying potential adverse effects early in the development pipeline. Mitochondrial toxicity, in particular, can have profound implications for cellular health and is implicated in various diseases and drug-induced toxicities.

At Creative Biolabs, we excel in providing comprehensive cell-based mitochondrial toxicity analysis services. Backed by years of expertise and a commitment to excellence, we offer a suite of specialized assays tailored to assess the impact of compounds on mitochondrial function with precision and reliability. Our dedication to advancing toxicity analysis ensures that you receive actionable insights that are essential for informed decision-making in drug discovery and development.

Our Services

Creative Biolabs offers a range of services for cell-based mitochondrial toxicity analysis, employing advanced methodologies and state-of-the-art technologies, specifically tailored for HepG2 cells:

• Mitochondrial Function Assays
  • Mitochondrial Membrane Potential Assessment: Using fluorescent dyes such as JC-1 or TMRM, we evaluate changes in mitochondrial membrane potential in HepG2 cells. This indicator provides crucial insights into mitochondrial health and function.
  • ATP Production Measurement: Luminescent or fluorescent assays are employed to assess ATP levels in HepG2 cells, providing a direct measure of mitochondrial energy production and overall cellular metabolism.
  • Oxygen Consumption Rate (OCR) Analysis: We quantify OCR in HepG2 cells to evaluate mitochondrial respiration and function under various conditions, offering valuable insights into mitochondrial bioenergetics.
• Mitochondrial Morphology Evaluation
  • Fluorescence Microscopy: High-resolution imaging of HepG2 cells allows for the visualization and analysis of mitochondrial morphology changes. This includes assessing mitochondrial fragmentation, elongation, and swelling, which serve as crucial indicators of mitochondrial dysfunction.
• Reactive Oxygen Species (ROS) Detection
  • DHE or DCFDA Staining: Fluorescent probes are utilized to detect intracellular ROS levels in HepG2 cells. This enables the assessment of oxidative stress-induced mitochondrial damage, providing insights into the potential cytotoxic effects of compounds on mitochondrial function.

Fig.1 Examples of HepG2 response to treatment with different compounds with various types of quantitative analysis.¹

Applications

• Drug Safety Assessment: Evaluate the mitochondrial toxicity of drug candidates in HepG2 cells to mitigate safety risks and optimize drug development strategies.
• Disease Modeling: Investigate the role of mitochondrial dysfunction in various diseases using HepG2 cells as a model system, including neurodegenerative disorders, metabolic diseases, and cancer.
• Toxicology Screening: Screen compound libraries in HepG2 cells to identify potential mitochondrial toxicants and prioritize compounds for further development.

Advantages

• Our assays offer multifaceted insights into mitochondrial function, morphology, and oxidative stress in HepG2 cells, enabling a thorough evaluation of compound toxicity.
• Leveraging advanced technologies, we achieve high sensitivity and specificity in detecting subtle changes in mitochondrial health and function in HepG2 cells.
• We provide customized assay designs and flexible service options specifically optimized for HepG2 cells, ensuring the relevance and reliability of your data.

At Creative Biolabs, HepG2 cell-based mitochondrial toxicity analysis services offer precise and reliable assessments of compound-induced mitochondrial dysfunction. Contact us today to leverage our tailored solutions and unlock valuable insights into compound safety and efficacy.

Reference

1. Tsiper, Maria V., et al. "Differential mitochondrial toxicity screening and multi-parametric data analysis." (2012): e45226.

For Research Use Only | Not For Clinical Use