Cell Viability Measurement Service

Ensuring Cellular Health, Advancing Your Research

Cell viability measurement is a critical assay in various fields of biomedical research, drug development, and toxicity testing. It assesses the health and functionality of cells in response to various treatments or conditions. The primary goal is to determine the number of living, healthy cells within a population, providing insights into the cytotoxic effects of compounds, environmental stresses, or genetic modifications.

HepG2 is a well-established human liver cancer cell line derived from hepatoblastoma. It is widely used in scientific research due to its stable phenotype and the ability to retain many characteristics of normal liver cells. Here are the key features of HepG2 cells:

• Functionality: Exhibits many liver-specific functions, including the production of various plasma proteins and enzymes.
• Research Applications: Extensively used in drug metabolism and hepatotoxicity studies, liver disease research, and cancer biology.
• Advantages: Easy to culture, high transfection efficiency, and responsiveness to various treatments make HepG2 an ideal model for in vitro studies.

At Creative Biolabs, we provide a comprehensive mobile viability dimension carrier for the usage of cutting-edge strategies and technology. With a dedication to excellence, we provide tailor-made answers to aid an extensive variety of research targets, especially inside the ADME framework vital for drug development. Our cellular viability service employs a functional assay technique, in particular, designed for cellular-primarily based evaluation. Using HepG2 cells, we utilize luminescence-based total detection techniques to assess cellular viability with extraordinary sensitivity and accuracy. This approach allows a complete analysis of drug consequences, toxicity profiles, and mobile responses. Cell Viability Measurement is important for researchers aiming to recognize the effect of compounds on liver cells and to increase more secure and greater effective tablets.

Technical Procedure:

1. Cell Culture: HepG2 cells are cultured and seeded in multi-properly plates beneath controlled situations.
2. Treatment: Cells are uncovered to test compounds, therapeutic agents, or experimental conditions of interest.
3. Incubation: After treatment, cells go through incubation to allow for mobile reactions.
4. Luminescence Assay: A luminescent substrate is brought to every properly to quantify ATP degrees, indicative of cellular viability.
5. Measurement and Analysis: Luminescence signals are measured using a microplate reader, and statistics are analyzed to determine mobile viability percentages and cytotoxicity effects.

Published Data

This study illustrates the effect of website-specific breathing chain (RC) inhibition on the viability of the HepG2 most cancers mobile line. The parent demonstrates that focused inhibition of diverse additives of the mitochondrial respiration chain extensively affects the viability of HepG2 cells. Inhibitors used in the have a look at include rotenone, whose objectives are complex I; antimycin A, targeting complicated III; and oligomycin, targeting ATP synthase (complex V). The effects reveal a marked decrease in cell viability following the utility of these inhibitors, indicating that mitochondrial characteristic plays an important function in the survival of HepG2 cells. This shows that disruptions in the breathing chain can efficaciously lessen cellular viability, highlighting potential therapeutic objectives for interventions geared toward impairing cancer cellular metabolism and proliferation. The findings underscore the significance of mitochondrial integrity in preserving the viability of HepG2 cells and offer insights into the mechanisms by which mitochondrial inhibitors exert their cytotoxic effects.

Fig.1 Effect of site-specific RC inhibition on the viability of HepG2 cancer cell line.¹

Applications

• Assessing drug efficacy and toxicity profiles in HepG2 cells.
• Screening compounds for their impact on HepG2 cellular viability.

Advantages

• Rigorous and established methodologies tailor-made for HepG2 cellular research
• Customizable assay protocols to satisfy specific study goals
• Expertise in dealing with HepG2 cells to ensure reliable and reproducible outcomes
• Rapid turnaround times with designated experimental reviews
• Comprehensive records analysis and interpretation help

Precision in Cell Viability Assessment

Our cellular viability size provider using HepG2 cells affords a reliable and applicable version for analyzing liver toxicity, drug metabolism, and most cancer therapeutics. Discover the full potential of our cellular viability dimension provider at Creative Biolabs. Whether you're carrying out drug improvement, toxicity tests, or fundamental studies concerning HepG2 cells, our team is dedicated to advancing your scientific discoveries. Contact us today to talk about how cellular viability measurement provider services can pressure innovation in biomedical sciences.

Reference

1. Doczi, Judit, et al. "Viability of HepG2 and MCF-7 cells is not correlated with mitochondrial bioenergetics." Scientific Reports 13.1 (2023): 10822.

For Research Use Only | Not For Clinical Use