CellOnco™ CHO-K1-Tg(Human M2 Receptor) Division-Arrested Cell, Single Clone (CAT#: ITS-0624-HMM627)

Creative Biolabs offers CHO-K1-Tg(Human M2 Receptor) Division-Arrested Cell which M2 receptor stably expressed in CHO-K1 cells.

CHO-K1-Tg(Human M2 Receptor) Division-Arrested Cell was engineered to express the receptor human M2 (NM_001006632.1). This cell line can be used to study M2 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

M2 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

M2

Muscarinic

Human

CHO-K1

Expression vector containing full-length human CHRM2 cDNA (GenBank Accession Number NM_001006632.1) with FLAG tag sequence at N-terminus

NM_001006632.1

The muscarinic M2 receptor is a 466-amino acid, 7-transmembrane protein. In heart muscle, M2 receptors represent the prevailing subtype of muscarinic receptors. They can also be found in neurons of central and peripheral nervous system. In neuronal cells, mainly on synaptic terminals, stimulation of the M2 autoreceptors is responsible for presynaptic muscarinic autoinhibition of acetylcholine release in both central and peripheral cholinergic neurons. Loss of function of these M2 receptors, as occurs in some patients with asthma and in animal models of inflammation, leads to an increase in vagally mediated hyperreactivity.