CHO-K1-based OATP1B1 Substrate Assessment Service
As a liver-specific transporter, OATP1B1 plays a key role in the uptake of various drugs and endogenous compounds by hepatocytes. In order to assess a compound as a substrate for OATP1B1, a commonly used in vitro model involves using Chinese
hamster ovary (CHO) cells expressing the human OATP1B1 transporter. Creative Biolabs utilizes the CHO-K1 cell line to assess the permeability of compounds through OATP1B1.
OATP1B1
OATP1B1 (organic anion-transporting polypeptide 1B1) is a liver-specific transporter protein that is responsible for the uptake of various endogenous and exogenous compounds into hepatocytes. It is known to transport a wide range of substrates, including
bile acids, steroid hormones, and a variety of drugs and drug metabolites.
CHO-K1-based OATP1B1 Substrate Assessment Service
Our CHO-K1-based OATP1B1 substrate assessment service is designed to evaluate
the substrate specificity of compounds for OATP1B1, a key transporter involved
in the uptake of various drugs and endogenous compounds. Using a cell-based
assay system, we measure the transport of your compound by OATP1B1 and
determine its potential to interact with this transporter. By assessing the
substrate potential of your compound for OATP1B1, you can gain valuable
insights into its pharmacokinetic properties and potential drug-drug
interactions. This information can help guide further development of your
compound and optimize its therapeutic potential. Here's the specific
information about this service:
Assay Information
Construct Details
Full length, recombined
Assay Sub Type
Cell Based
Process of Our Service
1
Generation of OATP1B1-CHO Cells
CHO cells can be transfected with a plasmid containing the gene encoding human OATP1B1 to create stable OATP1B1-CHO cell lines.
2
Uptake Assay
The OATP1B1-CHO cells can be incubated with the test compound at a relevant concentration. Samples can be taken at various time points to measure the intracellular accumulation of compounds. The uptake is measured using radioactively labeled compounds
or LC-MS/MS quantitation method.
3
Competitive Inhibition Assay
The substrate potential of the test compound can be further confirmed by performing competitive inhibition studies using known OATP1B1 substrates.
4
Inhibition studies
Transporter inhibitors can be used as positive controls to show the specificity of the uptake by OATP1B1
5
Data Analysis
The uptake data can be analyzed to determine the kinetic parameters (e.g. Km and Vmax) of the test compound for OATP1B1.
Prospect of Our Service
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Reliable assessment of OATP1B1 substrate properties
CHO-K1 cells have been widely used as a model system for studying drug transporters, including OATP1B1. Utilizing these cells for substrate assessment can provide valuable
information on the uptake properties of a compound.
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Cost-effective screening tool
Using CHO-K1 cells for OATP1B1 substrate assessment can be a cost-effective and efficient way to screen potential drug candidates early in the drug development process.
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Accelerated drug development
By efficiently identifying OATP1B1 substrates early in the drug development process, researchers can optimize candidate compounds and accelerate the development of new drugs.
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Potential for customized assay development
The CHO-K1-based OATP1B1 substrate assessment service may offer flexibility in assay development, allowing for customization based on specific research needs.
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Enhanced understanding of drug transporter interactions
Studying the interactions between compound molecules and OATP1B1 can provide
valuable insights into their pharmacokinetics and potential drug-drug
interactions.
Frequently Asked Question
A1: What is the timeframe for the CHO-K1-based OATP1B1 substrate evaluation
service?
Q1: The timeframe for the CHO-K1-based OATP1B1 substrate evaluation service
can vary depending on the specific requirements of the study and the
availability of resources. Generally, the evaluation service can be completed
within 4-6 weeks, depending on the complexity of the study design and the
number of samples that need to be processed. It is recommended to contact us
for a more accurate estimate of the timeframe for a particular evaluation.
Contact Us
Our experienced team of scientists can customize our assay to meet your
specific needs and provide reliable data on the substrate specificity of your
compound for OATP1B1. Contact us now to learn
more about our CHO-K1-based OATP1B1 substrate assessment service and how we
can support your drug development efforts.
For Research Use Only | Not For Clinical Use
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