CHO-K1-based OATP1B3 Substrate Assessment Service
OATP1B3 is an important transporter protein that plays a key role in the uptake of various drugs and compounds into cells. In order to assess whether a particular compound is a substrate for OATP1B3, a common method involves using a cell line that overexpresses
the OATP1B3 protein (OATP1B3-CHO cells) and measuring the uptake of the compound into these cells.
OATP1B3
Organic Anion Transporting Polypeptide 1B3 (OATP1B3) is a protein that is encoded by the SLCO1B3 gene in humans. It is a member of the solute carrier organic anion transporter family and is primarily expressed in the liver, where it plays a key role in
the uptake of various endogenous compounds and drugs into hepatocytes. OATP1B3 is responsible for the transport of a wide range of substrates, including bile acids, hormones, and various drugs and toxins. Its function in the liver is essential
for maintaining proper bile acid homeostasis, as well as for the elimination of drugs and toxins from the body.
CHO-K1-based OATP1B3 Substrate Assessment Service
Our CHO-K1-based OATP1B3 Substrate Assessment Service is designed to evaluate the substrate potential of your compound towards the OATP1B3 transporter. CHO-K1 cells overexpressing the OATP1B3 transporter will be used as a model system for this assessment.
By utilizing our OATP1B3 Substrate Assessment Service, you can gain valuable insights into the potential interaction of your compound with the OATP1B3 transporter. This information can help guide further development of your compound and optimize its
pharmacokinetic properties. Here's the specific information about this service:
Key Features
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Utilization of stable CHO-K1 cell lines overexpressing the OATP1B3 transporter
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Assessment of transport kinetics and substrate specificity of your compound towards OATP1B3
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High-throughput screening capabilities for efficient evaluation of multiple compounds
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Detailed report with comprehensive data analysis and interpretation
Assay Information
Construct Details
Full length, recombined
Assay Sub Type
Cell Based
Process of Our Service
To test whether a compound is a substrate for OATP1B3, our service typically incubates the compound with OATP1B3-CHO cells and monitors its intracellular accumulation over time. If the compound is indeed a substrate for OATP1B3, it would be expected to
be taken up by the cells in a time-dependent manner and show higher intracellular levels compared to control cells that do not express OATP1B3. In addition to uptake studies, we may also conduct inhibition studies to further confirm the specificity
of the interaction between the compound and OATP1B3. This can involve pre-treating the OATP1B3-CHO cells with specific OATP1B3 inhibitors and then testing the uptake of the compound to see if it is reduced in the presence of the inhibitors.
- Preparation of the OATP1B3-CHO cells
- Incubation with the compound
- Measurement of substrate uptake
- Data analysis
- Interpretation of results
Frequently Asked Question
A1: Why do CHO-K1-based OATP1B3 substrate assessment?
Q1: In the context of drug development, understanding whether a compound is a substrate for OATP1B3 can help predict its pharmacokinetic behavior, such as its absorption, distribution, metabolism, and excretion (ADME). This information is crucial for
determining the compound's bioavailability, efficacy, and potential for drug-drug interactions. Assessing OATP1B3 substrate activity in a cellular system, such as OATP1B3-CHO cells, allows researchers to study the specific interactions between the
compound and the transporter in a controlled environment. This can help identify potential inhibitors or inducers of OATP1B3 activity and guide the development of safer and more effective drugs.
Contact Us
Contact us now to learn more about our CHO-K1-based OATP1B3 Substrate Assessment Service and how it can benefit your research and development efforts.
For Research Use Only | Not For Clinical Use
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