CRISPR/Cas recombineering is the most powerful methodology for engineering bacterial genomes to date. Creative Biolabs is pleased to introduce the microbial genome editing service for knock-in, knock-out, or gene replacement in bacteria.

Background

Gut bacteria and the host live in a commensal manner. The gut microbiota plays a significant role in affecting the well-being of its host. However, gut bacteria can be potentially harmful when the ecosystem undergoes abnormal changes. Therefore, they can be used as a novel target to prevent and treat gut bacteria-related diseases. CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) system can be repurposed to selectively target and destruct pathogen genomes. The development of CRISPR/Cas9 as an accurate and versatile gene-editing technology has led pathologists to explore its disease-mitigation applications in both hosts and pathogens. The development of CRISPR/Cas9 as an accurate and versatile gene-editing technology has led pathologists to explore its disease-mitigation applications in both hosts and pathogens.

CRISPR Services

CRISPR technologies are the most powerful methodology for engineering bacterial genomes to date. CRISPR-Cas9 is part of bacterial immune systems and it is now being used to reverse-engineer bacteriophages to be better at attacking and killing pathogenic bacteria. Engineered CRISPR-Cas systems can be used to control gene expression and modulate the production of metabolites and proteins. Creative Biolabs provides CRISPR service for targeted pathogens by several methods. CRISPR-Cas9-based methods vary on the number of plasmids used, the use of heterologous recombinase (e.g., lambda Red), and the DNA repair mechanism induced. We aim to offer customizable and flexible services and to fit how you run projects.

Fig.1 Strategies used for CRISPR-Cas based genome editing in bacteria. (Arroyo-Olarte, 2021)

We provide professional microbial genome editing services including CRISPR design, testing, and production services. Our well-designed system potentially only targets pathogens while preserving commensal bacteria in the microbiota.

As a premier company in microbial genome editing, Creative Biolabs employs a powerful and flexible gene-editing platform and a series of services for genetic modification which has been successfully achieved in microorganisms. Our talented scientists have extensive experience in gene knockout, knock-in/replacement, and site-directed mutagenesis. Our CRISPR editing services include highly selective and programable bacterial killing as well as modification of the human microbiome via gene modification and the expression of therapeutic proteins or peptides. A series of complete solutions for genome editing help you every step of the way in your genome editing workflow. Our experience and expertise ensure that your project will meet your specific needs. If you are interested in our services, please feel free to contact us.

Reference

1. Arroyo-Olarte, R.D.; et al. Genome editing in bacteria: CRISPR-Cas and beyond. Microorganisms. 2021, 9(4): 844.

For Research Use Only | Not For Clinical Use