Scientists at Creative Biolabs apply molecular principles to conceive, design, and build synthetic gene circuits that effectively carry out therapeutic tasks by genetically modifying cancer cells. We are dedicated to engineering 22Rv1 cells utilizing several strategies to meet your project needs.

Introduction of 22RV1 Cell Line

22Rv1 is a human prostate carcinoma epithelial cell line established from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft in the late 1990s. 22Rv1 cell expresses mutant (H874Y) androgen receptors (AR) and secretes low levels of prostate-specific antigen (PSA). It is an androgen-independent prostate cancer cell but responds to androgens. 22Rv1 cells can both respond to hormones and display hormone-independent growth. As the most aggressive prostate cancer cell line, the 22Rv1 cell line represents an AR-positive prostate cancer model to study aspects of prostate cancer tumorigenesis in vivo and is a commonly used preclinical model of prostate cancer.

• Characteristics of 22RV1 Cell Line

Engineering 22RV1 Cell Line Service

Prostate cancer is a biologically heterogeneous disease and its complex nature provides a significant challenge for its clinical management. Immunotherapy for prostate cancer works by stimulating a person's own immune system to recognize and destroy cancer cells more effectively. Gene-modified tumor cell has revolutionized the gene therapy field though there are some difficulties and limitations. Creative Biolabs offers engineering cancer cell line service for a wide variety of genes. We can engineer custom 22RV1 cell lines with a wide gene-modification of gene-modification to speed up your immunotherapy studies.

• Gene Delivery Methods

Gene delivery systems are essentially necessary for the gene therapy of human genetic diseases. One of the approaches that can be undertaken is the use of viral/non-viral vectors, to modify tumor cells and thereby activate and direct immune responses against tumor cells. Equipped with an exhaustive portfolio of genetic techniques and tools, we can tailor the best-possible genetic design. We possess a series of cell line construction technologies including CRISPR technology, lentivirus infection, and retrovirus infection, thus shortening your project turnaround time.

• Strategies for Engineering 22RV1 Cell

Many different novel strategies have been developed to derive genetically modified tumor cells and use them as cellular vaccines to induce useful antitumor immunity in a variety of animal tumor models. Many cytokines and costimulatory molecules have been transfected in a number of tumor cells and in several cases, these gene-modified tumor cells, when used as vaccines, induced tumor immunity leading to rejection of a challenge with the parental tumor. In addition, we also provide other off-the-shelf strategies customized to engineer 22Rv1 cells.

• One-stop Services

Please feel free to contact us, our scientists will tailor the most reasonable plan for your project.

Reference

1. Beitz, A.M.; et al. Synthetic gene circuits as tools for drug discovery. Trends in Biotechnology. 2022, 40(2):210-25.

For Research Use Only | Not For Clinical Use