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IntroductionMicroarray vs. RNA-seqAnalysis PipelineBrochure
Gene expression profiling is a powerful technique used to analyze gene activity across thousands of cells simultaneously, providing a global view of cellular function. This method allows for the identification of
new drug targets by comparing gene expression between treated and untreated cells, facilitating the evaluation of treatment-related phenotypic changes at the molecular level. Gene expression profiling is particularly valuable for studying
the tumor microenvironment, identifying target cells, and advancing drug discovery.
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Creative Biolabs offers comprehensive gene expression profiling services, leveraging extensive expertise across molecular biology, genetics, and bioinformatics. Our team is proficient in advanced techniques such as DNA microarrays, transcriptomics sequencing, digital PCR, and real-time PCR, enabling us to provide customized solutions tailored to each client’s project needs. We focus on selecting and optimizing the most appropriate technology for
each research goal, ensuring accurate and high-quality results.
Microarray Analysis vs. RNA-seq Analysis
Aspect
Microarray Analysis
RNA-seq Analysis
Technology
hybridization-based techniques
predefined probes
sequencing technology
capture and quantify RNA transcripts
Platforms
Affymetrix - a pioneer in microarray technology
Agilent - an integrated, flexible, open approach
Illumina - a highly reproducible, multiplex efficient, and cost-effective option
Next-generation sequencing (NGS)
Single cell sequencing
In situ sequencing of fixed tissue
Applications
Suitable forgene expression studies that focus on:
Well-annotated genomes
Predefined targets
Suitable for discovering novel genes:
Novel genes discovery
Alternative splicing events
Entire transcriptomes analysis
Scope of Analysis
Narrow, limited to known genes or transcriptsthatpresent in the microarray probes
Broad, both known and novel transcripts, including isoforms and alternative splicing
Data Output
(Whether predefined probes are required?)
Yes, it provides relative gene expression levels based on predefined probes
No, it offers absolute quantification of RNA transcripts without relying on predefined probes.
Sensitivity
Suitable for high-abundance transcripts
High sensitivity forlow-abundance transcripts andnovel RNA species
Microarray analysis and RNA-seq analysis can be applied to a wide range of organisms, including humans, mice, and rats. Microarray analysis is ideal for quick and cost-effective studies where the focus is on known gene expression
profiles, while RNA-seq analysis offers deeper insights, detecting novel transcripts and providing base-pair resolution, making it suitable for more comprehensive and exploratory studies. Creative Biolabsis professional in recommending the best-fit solutions according to your specific requirements. If you hesitate about choosing the more suitable method and want to get more customized suggestions for your research, please feel free to contact us.
Analysis Pipeline
Array Data Extraction
↓Detectable Signal
Data Normalization
↓Quantile
Statistical Testing
↓P-value Calculation
RNA-seq Analysis
↓Test Enrichment calculation
Final Report
Why Choose Us?
Applicable to various species
Tailored solutions for humans, mice, rats, and other model organisms.
Streamlined process
Standard genome-wide expression analysis for you.
Custom array design and validation
Tailored solutions from method determination to final analysis.
Professional support
Expert technical support throughout the project to ensure high repeatability, efficiency, and cost-effectiveness.
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