H4-II-E-C3-based Cytotoxicity Safety Screening Service
Novel Compounds & Drug Candidate Safety
The evaluation of novel compounds for toxicity is an indispensable step in drug development, ensuring the safety and efficacy of potential drug candidates. Cytotoxicity testing focuses on evaluating the potentially harmful effects that new compounds may have on mammalian cells, ensuring only the safest candidates advance to clinical trials. The rat H4-II-E-C3 cell line is particularly advantageous for these assessments due to its well-characterized physiological relevance and consistent response to toxic insults, providing a reliable model for initial toxicity screening.
Why Choose the H4-II-E-C3 Cell Line?
H4-II-E-C3 cells, a clonal derivative of the rat hepatoma H4-II-E line, are an excellent choice for cytotoxicity testing owing to their intrinsic characteristics that closely mimic the metabolic functions of human liver cells. These cells exhibit stable, well-characterized growth and metabolic profiles, making them highly reliable for reproducible results. As a hepatoma cell line, H4-II-E-C3 possesses robust enzymatic activity. This metabolic proficiency makes them especially valuable for evaluating the hepatotoxic potential of various compounds. Furthermore, their ease of culture and maintenance, coupled with their sensitivity to a wide range of toxicants, enables precise and efficient toxicity assessments. At Creative Biolabs, we employ H4-II-E-C3 cells in cytotoxicity assays to obtain crucial data that inform the safety and potential side effects of new therapeutic agents.
H4-II-E-C3-based Cytotoxicity Screening Services
Creative Biolabs offers comprehensive H4-II-E-C3-based cytotoxicity screening services aimed at evaluating the cytotoxic potential of compounds. Using advanced cell viability assays, our service provides a reliable and efficient method for determining compound toxicity in H4-II-E-C3 cells. This approach allows for a high-throughput screening capability, facilitating the fast and accurate identification of potentially toxic candidates early in the drug development process.
Cell Viability Screening & ATP Content Measurement
We utilize a robust cell viability screening method to measure the cytotoxicity of compounds on H4-II-E-C3 cells, focusing on the quantification of host cell ATP content as an indicator of cell health. The assay operates by detecting the presence of ATP, which correlates with metabolically active and viable cells. The luminescent readout provides a sensitive and accurate measure of cell viability, allowing for precise determination of cytotoxic effects. Our stringent normalization and assay interpretation protocols ensure that the data generated is reliable and reproducible, facilitating the accurate assessment of compound safety.
Assay Overview
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Target Type
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H4-II-E-C3 cell toxicity
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Assay Type
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Cell viability
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Substrate
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Beetle luciferin + ATP
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Detection Type
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Luminescence
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Cell Type
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H4-II-E-C3 cell line
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Incubation Time
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48 hours at 37°C. Tailored by clients available
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Test Concentration
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To be determined
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Advantages of Our Service
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High Throughput Screening: Ability to handle a large volume of samples efficiently.
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Relevance: The use of H4-II-E-C3 hepatoma cells aligns closely with human liver metabolism, offering insightful data relevant to human health.
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Consistency: Reliable and reproducible results ensure robust data for decision-making processes.
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Sensitivity: Accurate measurement of ATP content provides a sensitive readout of cellular health and viability.
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Comprehensive Analysis: Detailed assays and normalization techniques offer a thorough understanding of compound toxicity.
For further assistance or additional information, please feel free to contact us.
For Research Use Only | Not For Clinical Use
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