High Content Screening Assay - Creative Biolabs

High content screening (HCS) is an analytical tool that uses microscopic imaging to observe, measure, and analyze multiple cellular parameters in a bioassay. By applying fluorescent dyes, fluorescent labeled proteins and antibodies, changes occurring within the cell can be detected and quantified after adding corresponding compounds and ligands.

As a long-recognized business in the field, Creative Biolabs specializes in developing cell-based high-content screening assays that can be tailored to your drug discovery process.

High Content Screening

High Content Screening Pipeline

Our extensive experience in high-content screening, internal image analysis algorithm design and disease biology allows us to offer more than 100 HCS analyses for our customers to choose from.

Example Assays Include

Apoptosis
Angiogenesis
Fat formation
Cell-cell junction and cadherin
Cell migration
Lipolysis
MAP kinase pathway
Mitochondrial health
Neural crest differentiation
Neural crest migration
Neuronal differentiation
Neurological function

Steatosis
Wnt signal
Autophagy and protein aggregation
Cell movement and migration
Cytoskeletal rearrangement
Epigenetic modification
Real-time imaging (e.g., Ca flux)
Marker expression
Protein acetylation and phosphorylation
Receptor internalization and degradation
Subcellular localization
Transcription factor translocation
Virus replication

Our HCS Features

For small scale screens:

Training / Use of HCS instruments
Training / Use analysis software
Use image analysis methods to perform selection, development, and/or validation
Optimize assay read-outs

For large scale screens:

Optimize assay biology turnouts
Develop algorithms and readings
Perform measurement miniaturization / verification
Use HTS or functional genomics facilities to screen

For more details, please feel free to contact us or send us a query directly.