HPLC-MS/MS-based Cytochrome P450 1A (CYP1A) Inhibition Assessment Service
Creative Biolabs offers comprehensive services to evaluate the impact of compounds on CYP1A activity, providing accurate and reliable data for drug development and safety assessment. Our experienced scientists will work closely with customers to design and execute custom assays, analyze results, and provide detailed reports to aid in decision-making. Trust us to deliver high-quality data and support for your research needs.
HPLC-MS/MS-based CYP Inhibition Assessment Platform
CYP1A in Disease
CYP1A refers to a subfamily of enzymes known as cytochrome P450 1A, which play a crucial role in the metabolism and detoxification of various substances in the body. These enzymes are mainly found in the liver, where they help to break down and eliminate drugs, toxins, and other foreign compounds that enter the body. CYP1A enzymes are also involved in the metabolism of certain endogenous compounds, such as steroids and fatty acids. Mutations or variations in the CYP1A genes can affect an individual's ability to metabolize drugs and other substances, and may influence their risk of developing certain diseases.
HPLC-MS/MS-based Cytochrome P450 1A (CYP1A) Inhibition Assessment Service
Our experienced team utilizes state-of-the-art HPLC-MS/MS technology to measure the activity of CYP1A in the presence of test compounds. This allows us to accurately determine the inhibitory effects of compounds on CYP1A activity, providing valuable information for drug development and safety assessment.
What We Do
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Evaluation of test compounds for their potential to inhibit CYP1A activity
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Determination of IC50 values for CYP1A inhibition
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Comprehensive analysis and interpretation of data
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Detailed report outlining results and recommendations
Detailed Information of Our Service
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Protein Name
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Family
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Sub Family
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Uniprot Number
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Gene Name
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CYP1A1
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CYP450
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CYP1
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P04798
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CYP1A1
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Gene ID
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Gene Aliases
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Organism
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Construct Details
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Tissue
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1543
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P450DX|P1-450|P450-C|CP11
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Human
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Microsomes
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Liver
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Measured Response
Peak Area Response
Service Sub Type
Enzymatic
Detection Method
HPLC-MS/MS
Importance of CYP1A Inhibition Assessment
Assessment of CYP1A inhibition is important for predicting drug interactions, understanding drug metabolism, ensuring safety, and meeting regulatory requirements in drug development.
The CYP1A enzyme is involved in the metabolism of a variety of drugs and exogenous substances. Inhibition of these enzymes can lead to increased blood levels of these drugs, potentially resulting in toxicity or reduced efficacy.
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Predicting drug metabolism
Understanding and assessing the extent of CYP1A inhibition can help predict how drugs are metabolized in the body.
Inhibition of CYP1A enzymes can also lead to the formation of reactive metabolites, which can be toxic to cells and tissues. Assessing CYP1A inhibition helps to identify potential safety risks associated with drug candidates.
Regulatory agencies require an assessment of CYP1A inhibition as part of the drug development process.
Cooperation With Creative Biolabs
Frequently Asked Question
A1: Are there any other techniques to be used to assess cytochrome P450 1A (CYP1A) inhibition?
Q1: Yes, there are several other techniques that can be used to assess cytochrome P450 1A (CYP1A) inhibition. Some of these techniques include Fluorescence assays, Radiometric assays, and Molecular modeling. However, the assay is typically conducted using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology, which allows for the sensitive and accurate quantification of substrates and metabolites in biological samples. In this assay, test compounds are incubated with human liver microsomes or recombinant CYP1A enzyme, along with a CYP1A-specific substrate.
Contact Us
Our HPLC-MS/MS-based CYP1A inhibition assessment service is conducted by our team of expert scientists with years of experience in drug metabolism and pharmacology. We offer fast turnaround times and competitive pricing for related services to meet your R&D needs. Contact us now to learn more about our HPLC-MS/MS-based CYP1A inhibition assessment services.
For Research Use Only | Not For Clinical Use
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