Human Transporter MATE1 Fluorescence-based Antagonist Assay Service
This unit describes an assay for determining a compound's ability to inhibit the MATE1 transporter. MATE1 is a transporter protein that plays a crucial role in the efflux of organic cations across the cell membrane. Creative Biolabs has launched a high-throughput, reliable, and cost-effective solution for evaluating the inhibitory effects of compounds on the MATE1 transporter protein.
MATE1
MATE1 (Multidrug and Toxin Extrusion Protein 1) is a membrane protein that belongs to the solute carrier family of transporters. It is primarily involved in the transport of organic cations across cell membranes. MATE1 is found in various tissues in the body, including the kidney, liver, and intestines, where it plays a crucial role in the elimination of drugs and toxins from the body. In the kidney, MATE1 is responsible for transporting organic cations from the blood into the kidney tubules, where they can be excreted in the urine. This process helps regulate the levels of potentially harmful substances in the body and is essential for maintaining overall health and homeostasis.
Our Service
In our service, the MATE1 transporter is overexpressed in Human Embryonic Kidney (HEK) cells and a fluorescent substrate, such as 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (DAPI), is used to monitor the transport activity of MATE1. The assay involves incubating the MATE1-HEK cells with the fluorescent substrate in the presence of varying concentrations of the compound being tested. The fluorescent signal is then measured, and the extent of inhibition of the transport activity of MATE1 by the compound is determined.
Detection Method
Fluorimetry
Functional Mode
Antagonist
Measured Response
Fluorescence
Our service workflow involves the use of HEK cells overexpressing MATE1, incubation with the DAPI substrate, and measurement of fluorescence intensity to assess the inhibitory potential of test compounds against the MATE1 transporter.
1
Introduction of MATE1 inhibitor to HEK cells expressing MATE1 protein.
2
Inhibition of MATE1 transporter activity, preventing the transport of substrates.
3
Addition of DAPl substrate to the cell culture medium.
4
DAPl substrate is unable to be transported by MATE1 due to the inhibition.
5
Accumulation of DAPl substrate in the cell culture medium.
6
Measurement of fluorescence intensity of accumulated DAPl substrate to evaluate the degree of MATE1 inhibition.
7
Analysis of the results and data to determine the effectiveness of MATE1 inhibitor.
Applications
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Identify potential drug candidates that could be used to treat various medical conditions.
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Study MATE1 inhibition can help in understanding drug interactions and predicting potential adverse effects when multiple drugs are taken simultaneously.
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Alter the pharmacokinetic parameters of the drug, which leads to a change in its efficacy and safety.
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Assess the potential toxicity of drugs and other substances.
Why Choose Creative Biolabs
Contact Us
If you have any inquiries or require further information regarding MATE1 inhibition, particularly in the context of MATE1-HEK and DAPl substrate research, please do not hesitate to contact us. Our team of experts is dedicated to providing you with the necessary support and guidance to address any questions or concerns you may have.
For Research Use Only | Not For Clinical Use
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