Human Transporter MATE2-K Fluorescence-based Inhibition Assay Service
MATE2-K is a transporter protein that plays a role in the elimination of various drugs and toxins from the body. Inhibition of MATE2-K can have implications for drug metabolism and potentially lead to drug interactions or altered drug efficacy. Here, Creative Biolabs offers a high-throughput, reliable, and cost-effective solution for evaluating the inhibitory effects of compounds on the MRP2-K transporter protein.
MMATE2-K
The MATE2-K inhibition assay involves using HEK293 cells that are engineered to overexpress the MATE2-K protein. These cells are then exposed to a fluorescent substrate, such as a drug-like compound known as DAPl, which is a substrate for MATE2-K. By measuring the fluorescence emitted by the DAPl substrate, we assess the activity of MATE2-K in the presence of various compounds. Inhibitors of MATE2-K will reduce the efflux of the DAPl substrate, leading to higher intracellular levels of the fluorescent compound. By identifying and characterizing MATE2-K inhibitors, we help you improve the safety and efficacy of pharmaceutical compounds.
Our Service
The MATE2-K inhibition assay involves using HEK293 cells that are engineered to overexpress the MATE2-K protein. These cells are then exposed to a fluorescent substrate, such as a drug-like compound known as DAPl, which is a substrate for MATE2-K. By measuring the fluorescence emitted by the DAPl substrate, we assess the activity of MATE2-K in the presence of various compounds. Inhibitors of MATE2-K will reduce the efflux of the DAPl substrate, leading to higher intracellular levels of the fluorescent compound. By identifying and characterizing MATE2-K inhibitors, we help you improve the safety and efficacy of pharmaceutical compounds.
Assay Sub Type
Cell-Based
The workflow of our service includes incubating test compounds with MATE2-K transporter-expressing cells, measuring fluorescence intensity levels to assess transporter activity, and analyzing the inhibitory effects of compounds on transporter function.
1
Sample preparation
Clients provide their samples to us for analysis.
2
Assay setup
We set up the fluorescence-based inhibition assay using the human transporter MATE2-K protein.
3
Inhibitor screening
The samples provided by the client are added to the assay wells containing the human transporter MATE2-K protein and the fluorescent substrate.
4
Data acquisition
The fluorescence signal from the assay wells is measured using a plate reader or a similar instrument.
5
Data analysis
We analyze the data obtained from the inhibition assay to determine the potency and efficacy of the tested compounds as inhibitors of the human transporter MATE2-K.
6
Reporting
The client is provided with a detailed report summarizing the results of the inhibition test.
7
Follow-up
We also offer additional services or support for follow-up studies.
Applications
This service offers high sensitivity in detecting interactions between potential inhibitors and MATE2-K, enabling accurate and reliable results.
Creative Biolabs provides fast results, allowing for quick evaluation of the inhibitory effects of compounds on MATE2-K.
The service is cost-effective, offering a cost-efficient method for screening potential inhibitors of MATE2-K.
The entire service process is performed by our experienced scientists who can provide expert guidance and support throughout the testing process.
The assay service can be tailored to suit different research needs and requirements, providing a flexible and customizable approach to inhibitor screening.
Creative Biolabs provides thorough data analysis and interpretation, helping researchers to understand the results and draw meaningful conclusions.
Other Services at Creative Biolabs
We are a leading provider of metabolite identification services, utilizing state-of-the-art technology and expertise to accurately identify and characterize metabolites in various biological samples. Our team of experienced scientists employs cutting-edge techniques such as LC-MS/MS and NMR spectroscopy to provide comprehensive metabolite profiling and elucidation.
Contact Us
For more information on MATE2-K inhibition services and how they can benefit your research projects, please contact us now. Our team of experts is ready to assist you in designing customized studies and experiments to meet your specific research needs.
For Research Use Only | Not For Clinical Use
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