Description
This kit has been developed to isolate highly purified human untouched B cells from human mononuclear cells (MNCs) by negative isolation in 30 minutes. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Features
No magnet, no column, no harsh chemicals.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and removed.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
Non-B cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD2, CD3, CD14, CD15, CD16, CD36, CD43, CD56, CD57, CD123, CD161, CD235ab, and CD314.
Method Type
Negative selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Datasheet