I-O™ Leukemic Cell Culture Kit (CAT#: ITS-0622-YT362)

1 kit

I-O™ CellExpander Hematopoietic Cells Medium II (SF), 100 mL
I-O™ CD34+ Expansion Supplement (10X), 10 mL
I-O™ UM-729, 250 µg

DMSO
Iscove's Modified Dulbecco's Medium (IMDM)
DNase I Solution
Trypan Blue
Tissue culture-treated plates
245 mm x 245 mm Square Treated Tissue Culture Dishes
35 mm Culture Dishes

Use sterile techniques to prepare I-O™ Leukemic Cell Culture Medium. The following example is for preparing 100 mL of medium. If preparing other volumes, adjust accordingly.
1. Thaw I-O™ CellExpander Hematopoietic Cells Medium II (SF) at room temperature (15 - 25°C) or overnight at 2 - 8°C. Mix thoroughly.
2. Thaw I-O™ CD34+ Expansion Supplement (10X) at room temperature (15 - 25°C) until just thawed. Mix thoroughly.
3. Add 10 mL of I-O™ CD34+ Expansion Supplement (10X) to 90 mL of I-O™ CellExpander Hematopoietic Cells Medium II (SF). Mix thoroughly.
4. Add 250 µg of UM-729 to 68 µL of fresh DMSO (final concentration 10 mM).
5. Prepare a 1 in 100 dilution of the 10 mM UM-729 stock solution by performing serial 1 in 10 dilutions.
6. Add 10 µL of diluted UM-729 per mL of medium to give a final concentration of 1.0 µM UM-729. Titration of UM-729 may be required to determine the optimal concentration for CD34+ cell expansion.

Leukemic Cell

Human

Liquid

When handling biohazardous materials such as human cells, safe laboratory procedures should be followed and protective clothing should be worn.

I-O™ CellExpander Hematopoietic Cells Medium II (SF) : Store at -20°C;
I-O™ CD34+ Expansion Supplement (10X), Store at -20 to -80°C;
I-O™ UM-729, Store at 15-25°C.

If not used immediately, aliquot into tubes and store at -20°C. After thawing aliquots, use immediately. Do not re-freeze.

Stable as supplied until expiry date on label.

Tested for sterility

Restrictions for research use only. Intended for use by laboratory professionals.