Immune Checkpoint Assays - Creative Biolabs

Immune checkpoints represent a group of membrane proteins expressed on effector cells (e.g., T/B cells and NK cells) that are composed of a variety of co-inhibitory and co-stimulatory pathways. They are involved in eliminating unwanted substances while maintaining their own tolerance, which plays a crucial role in immune regulation.

Generally, there are two types of immune checkpoints: activating receptors and inhibitory receptors. Thus, the two main mechanisms of immunotherapeutic antibodies can be defined as blocking the pathway (antagonists) and enhancing the pathway (agonists). To thoroughly validate the mechanism of action of these antibodies, Creative Biolabs has combined a range of cell-based assays with our state-of-the-art technology and in-depth knowledge in the field to provide a full range of immune checkpoints assay approaches for antibody functional characterization.

Immune Checkpoint Targets (including but not limited to):

Inhibitory Immune Checkpoints

Stimulatory Immune Checkpoints

Target	Receptor
B7-1(CD80)	PD-L1
B7-2(CD86)	CTLA4
PD-L1	PD-1
PD-L2	B7-1&PD-1
CD48	CD244
CD155	TIGIT
CD112	CD112R
KIR	MHC I
NKGD2	RAE-1

Target	Receptor
MHC	TCR
B7-H2	ICOS
CD137L	CD137
OX40L	OX40
GITRL	GITR
CD70	CD27
CD40	CD40L

Details of Our Checkpoint Assays

■ Immunological checkpoint blocking assays from multiple classical pathways, readings by FACS or ELISA
■ Custom cell-based, reporter or receptor-ligand assay
■ Induce biologically relevant responses without the use of difficult and expensive primary cells (reporter cell lines)
■ T cell proliferation assay, cytokine production assay, and T cell cytotoxicity assay
■ All tests are performed using high quality reagents and strictly controlled by experienced immunologists

We can help our clients design a roadmap that begins with the screening and analysis of the selected candidates, performs interaction analysis with the selected candidates, and then progresses to the analysis of the immunomodulatory function. Through this roadmap, we can demonstrate the binding and functional properties of antibodies and their underlying mechanisms of action.

For more details, please feel free to contact us or send us a query directly.