In our research on antibody-directed enzyme prodrug therapy (ADEPT), the in vitro experimental services offered by Creative Biolabs can provide significant support for your project. Our services are designed to help you precisely assess and optimize your ADEPT strategies, ensuring greater efficiency and reduced costs throughout your research and development process. We can efficiently evaluate the interactions between enzyme precursors and antibody complexes, as well as their efficacy in cellular models. Choose our services to elevate your ADEPT research to new heights and support the successful development of innovative drugs!

Introduction

In recent years, Creative Biolabs has launched a variety of specialized in vitro experimental services designed to provide CRO support for clients and accelerate the development of ADEPT-related therapies. Till now, we have offered expert antibody screening services that assist clients in selecting and optimizing suitable monoclonal antibodies based on the characteristics of target tumor cells. Based on high-throughput screening technologies, we can quickly assess the affinity and specificity of antibodies. Our team collaborates with clients to design and clone appropriate enzymes for expression and purification. Following this, we perform enzyme activity evaluations to ensure the effective conversion of prodrugs into active drugs in ADEPT applications.

In addition, we conduct ADEPT experiments using various tumor cell lines to evaluate the therapeutic effects of antibody-enzyme-prodrug combinations. Our services include quantitative analyses of multiple parameters such as cell proliferation, apoptosis, and migration, helping clients gain a comprehensive understanding of the treatment's impact on targets. Also, we will carry out studies on drug release kinetics to assess the intracellular release of prodrugs, explore their interactions with enzymes, and determine the bioavailability of the drug at different time points, all aimed at optimizing treatment strategies.

Fig.1 In Vitro Cytotoxicity Testing Assays.^1,3

Services

In the field of cancer treatment, ADEPT is emerging as an innovative targeted therapy that showcases its distinct advantages. To enhance the research capabilities of scientists and pharmaceutical companies in advancing ADEPT studies, Creative Biolab is happy to introduce a suite of in vitro experimental Services. These services include the binding affinity and specificity evaluation analysis, ADEPT antitumor analysis, and biological activity testing, all aimed at providing comprehensive support for your ADEPT research endeavors.

• Binding Affinity and Specificity Evaluation Analysis Service

In ADEPT research, the binding strength and specificity between antibodies and target antigens are critically important. Our binding affinity and specificity evaluation analysis service uses many biophysical techniques to accurately assess the affinity and specificity of antibodies toward target molecules through highly sensitive detection methods. Creative Biolabs consists of experienced scientists who can offer you extensive experimental designs and data analysis, facilitating a deeper understanding of antibody behavior and optimizing antibody design to improve treatment outcomes.

• Biological Activity Testing Service

In our labs, our biological activity testing services specialize in evaluating the efficacy of antibodies and their prodrugs in specific cell lines. Utilizing advanced technological platforms and rigorous experimental protocols, we deliver precise and dependable biological activity data. This service not only aids researchers in optimizing drug design but also provides crucial support for preclinical studies. Our team is composed of experienced scientists who can help with experimental designs based on client needs, ensuring that we meet the requirements of various research phases.

• ADEPT Antitumor Analysis Service

At Creative Biolabs, our ADEPT antitumor analysis Service focuses on evaluating the anticancer efficacy of ADEPT therapies against tumor cells. We use various cell models and quantitative analysis methods to systematically assess enzyme activity and its inhibitory effects on tumor growth post-drug conversion. Our service offers not only vital effectiveness data for targeted therapies but also provides scientific insights for exploring the potential of different drug combinations. Additionally, our experimental designs are adaptable to your specific needs and come with full reports to support your R&D efforts.

Fig.2 In Vitro Analysis Assays for Drug Efficacy Testing.^2,3

Procedure

At our company, the essence of ADEPT in vitro experimental services lies in its integration of cutting-edge biotechnology with efficient experimental design. By combining a range of techniques such as gene editing, recombinant antibody technology, and 3D cell culture, our services achieve more precise target localization and treatment. Furthermore, the use of biomimetic models enhances the clinical applicability of experimental results, effectively predicting in vivo responses.

STEP1: Target Selection and Validation

Using molecular biology techniques, our staff screen and validate potential target antigens. This process incorporates gene editing technologies (like CRISPR) to conduct functional studies on the antigens, confirming their expression and significance within tumor cells.

STEP2: Antibody Construction and Modification

Creative Biolabs uses recombinant DNA technology to construct monoclonal antibodies targeting specific antigens. Techniques for humanization are implemented to reduce immunogenicity and enhance therapeutic efficacy. High-throughput screening methods are used to quickly assess the affinity and specificity of antibodies, allowing us to select the best candidates.

STEP3: Biomimetic Model Development

In our labs, we create 3D cell culture models or organoids that closely mimic the in vivo microenvironment, enabling a more authentic simulation of interactions between antibodies and tumor cells, thus providing a reliable basis for further efficacy evaluations.

STEP4: Drug Release Mechanism Investigation

Creative Biolabs can assess the release and metabolism of antibody drugs within cells using labeling and tracking techniques (such as fluorescent tagging or radioactive isotopes), aiming to optimize the pharmacokinetic properties of the drugs and enhance their therapeutic effects.

STEP5: Combination Therapy Assessment

We design multiple combination therapy strategies and utilize analytical methods such as flow cytometry and ELISA to evaluate the synergistic effects of antibodies alongside other therapies, in search of the most effective treatment combinations.

Advantages

Our ADEPT in vitro experimental services combine a deep understanding of antibody-drug drug characteristics and cellular targets. We can go beyond traditional cell culture and drug screening that can provide optimized solutions for specific therapeutic targets. Meanwhile, Creative Biolabs incorporates top-class technologies such as high-throughput screening, single-cell analysis, and multiplex immunofluorescence to conduct well-mature analyses of the interactions between antibody drugs and cells. With these advanced methods, we can efficiently assess the affinity, specificity, and cytotoxicity of antibodies, thereby accelerating the drug development process. Additionally, we focus on biomarker discovery to lay a foundation for future clinical translation.

• Expert Team: Our research team comprises seasoned biologists and chemists with a solid theoretical foundation and extensive practical experience, ensuring that you receive high-quality experimental designs and data analysis.
• Customized Solutions: Creative Biolabs recognizes the uniqueness of each project, which is why we offer tailored experimental plans to meet your specific research needs and objectives.
• Validation of Optimized Solutions: Through our precise in vitro experiments, you can effectively validate optimized combinations of antibodies, enzymes, and prodrugs, enhancing therapeutic efficacy while minimizing side effects.
• Comprehensive Technical Support: Our services provide extensive technical assistance, covering everything from pre-experiment planning to post-experiment data interpretation, ensuring that you encounter no technical roadblocks throughout your research process.

To assist you in advancing your research and development of the ADEPT technology more efficiently, Creative Biolabs is pleased to introduce our exceptional in vitro experimental services. Whether you need help with antibody screening, purification, or enzyme functionality validation, we offer top-notch experimental conditions. We invite you to contact us at any time to discuss the challenges and requirements you may encounter in your ADEPT research.

References

1. Jablonská, Eva, et al. "Test conditions can significantly affect the results of in vitro cytotoxicity testing of degradable metallic biomaterials." Scientific Reports 11.1 (2021): 6628.
2. Åkerlund, Emma, et al. "The drug efficacy testing in 3D cultures platform identifies effective drugs for ovarian cancer patients." NPJ Precision Oncology 7.1 (2023): 111.
3. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use