Isolation and Cultivation of Targeted Intestinal Flora
Creative Biolabs provides a variety of services to support needs for microbiology research and development. Based on our gut microbiota platform, our scientists can help isolate and culture intestinal flora.
Background
Intestinal flora is defined as bacteria and other organisms that live inside the intestines. The gut microbiota of an individual contains trillions of microorganisms that participate in various physiological functions. There is a growing interest in the intestinal flora and human health and disease. Isolation and cultivation of intestinal flora have spurred great leaps in medicine, including the discovery of antibiotics, identifying pathogens that cause infectious diseases, and vaccine development. Different gut microbiota has different growth requirements. When culturing them in the laboratory, it is essential to take note of these conditions because these are needed to be replicated to successfully grow microbial cultures in the laboratory.
Fig.1 Solutions for intestinal flora at Creative Biolabs.
Our Services
With extensive research of microbiota and the art technology in this field, Creative Biolabs provides optional methods for your projects in gut microbiota isolation and cultivation.
Traditional isolation and cultivation
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The most common approaches of obtaining pure microbe cultures rely on colony streaking on agar plates or “dilution-to extinction” procedures. In the latter case, samples are serially diluted in microwell plates until no bacterial growth is observed. These traditional cultivation approaches are low-throughput and low efficiency and may yield biased sampling.
High-throughput isolation and cultivation
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Advances in culturing techniques include encapsulation of bacteria into microdroplets, diffusion chambers simulating the natural environment of the samples, microfabricated cultivation chips and design of more effective culture media with high-throughput identification of the cultured bacteria. This automated workflow significantly reduces hands-on time compared to traditional methods.
Flow cytometry-based isolation and cultivation
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Flow cytometry is well adapted for complex bacterial microbiota studies. Flow cytometry and bacterial cell sorting under anaerobic conditions provide an approach of targeted enrichment and culture of species of interest from fecal material with fast and reliable quantification. When used in conjunction with appropriate staining and associated controls, it gives a general overview of microbiota composition and variations in longitudinal studies.
Creative Biolabs offers complete solutions to isolate and culture intestinal flora. Our scientists are experienced in microbial isolation, cultivation, phenotypical characterizations. We also provide sequencing services for non-culturable gut microbes. Our laboratory scientists will work with you to find solutions that fit your budget and your specific research needs. If you are interested in our services, please don’t hesitate to contact us.
Reference
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Ji, B.; Nielsen, J. From next-generation sequencing to systematic modeling of the gut microbiome. Frontiers in genetics. 2015, 6: 219.
For Research Use Only | Not For Clinical Use
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