Creative Biolabs offers human breast cell MCF-7-based proliferation assays to assess the growth and proliferation of human breast cancer cells.

MCF-7 Cell

MCF-7-based Proliferation Assay at Creative Biolabs

In our assay, MCF-7 cells are treated with a luminescent reagent that is metabolized by the cells to produce light. The amount of light produced by MCF-7 cells is directly proportional to the number of viable cells present in the culture. By measuring the luminescence intensity at different time points, we track the growth and proliferation of MCF-7 cells in response to various treatments or stimuli.

Assay Information

Assay Procedure

Process details

Our MCF-7-based proliferation assay involves the following steps:

• Cell Culture

MCF-7 cells are cultured in an appropriate growth medium in a sterile tissue culture plate. The cells are maintained at optimal conditions in an incubator with controlled temperature, humidity, and CO₂ levels.

• Treatment

The cells are then treated with compounds or drugs of interest at varying concentrations to assess their effect on cell proliferation. Control wells without any treatment are also included.

• Luminescence Assay

After the incubation period, a luminescence assay is performed to measure the proliferation rate of the cells. This assay uses a luminescent substrate that is added to the cells, which produces light in proportion to the amount of ATP present in the cells. ATP is a molecule that is essential for cellular energy production and is often used as a marker for cell viability and proliferation.

• Data Analysis

Finally, the luminescence signal is quantified using a plate reader, and the data is analyzed to determine the effect of treatment on cell proliferation.

How Our Service Can Assist Your Project

The Human Breast Cell MCF-7-based Proliferation Assay Service can assist your project in the following ways:

• Screening for potential anticancer drugs

This assay can help identify compounds that inhibit the proliferation of MCF-7 breast cancer cells, which can be further developed as potential anticancer drugs.

• Assessing the efficacy of existing drugs

The assay can be used to evaluate the effectiveness of existing cancer treatments on MCF-7 cells, providing valuable information for drug development and personalized medicine.

• Studying mechanisms of cancer cell proliferation

By analyzing the proliferation of MCF-7 cells in response to different compounds or treatments, researchers can gain insights into the molecular mechanisms of cancer cell growth and identify potential therapeutic targets.

• Evaluating the toxicity of compounds

The assay can also be used to assess the toxicity of potential drug candidates on MCF-7 cells, helping to prioritize compounds for further development based on their safety profile.

Frequently Asked Question

Q1: Why use MCF-7 cells for the proliferation assay?

A1: MCF-7 cells are commonly used for proliferation assays because they are a human breast cancer cell line that exhibits hormone receptor positivity and can be easily cultured in vitro. Additionally, MCF-7 cells have been extensively characterized and are known to have a relatively stable growth rate, making them a reliable model system for studying cell proliferation. Furthermore, their hormone receptor status makes them particularly useful for studying the effects of hormone-based therapies on cell proliferation. Overall, MCF-7 cells are a well-established and widely used model system for studying cell proliferation in breast cancer research.

Contact Us

If you are interested in using luminescence detection methods for tumor cell proliferation assays, Creative Biolabs has the experience and advanced instrumentation to help you with this service. You can contact us directly to discuss your specific research needs and get more information about the testing options, pricing, and turnaround times.

For Research Use Only | Not For Clinical Use