As a dedicated biotech CRO, Creative Biolabs offers comprehensive support services to clients worldwide, particularly in the area of safety assessment for cancer treatments. Our state-of-the-art technologies and experienced team enable us to deliver high-quality mitochondrial function analysis services, assisting in the identification and evaluation of cardiotoxicity induced by anti-cancer drugs.

Introduction

Cardiotoxicity associated with cancer drugs has become a significant focus of research in recent years, especially as many novel anticancer therapies have emerged, drawing increasing attention to their potential effects on the cardiovascular system. Current studies indicate that mitochondrial function plays a crucial role in cellular energy metabolism, and anticancer medications may induce cardiotoxicity by disrupting mitochondrial function.

Creative Biolabs offers services for analyzing the mechanisms behind cardiotoxicity caused by anticancer drugs and mitochondrial dysfunction. We can systematically evaluate the effects of anti-cancer drugs on mitochondrial function. We can assess mitochondrial activity through the analysis of oxygen consumption rates and ATP production to understand how the drugs impact energy metabolism. Meanwhile, monitoring the mitochondrial membrane potential allows us to observe in real-time the effects of the drugs on the mitochondrial environment. Additionally, we can conduct dynamic analyses of mitochondria to study their fusion and fission states as well as morphological changes. Gene expression profiling will help identify alterations in genes related to mitochondrial function, shedding light on the mechanisms of the drug's action and potential cardiotoxicity. Up to now, by accurately delineating the influence of anti-cancer drugs on mitochondrial function, we can provide a scientific basis for new drug development and enhance patient safety.

Fig.1 Mitochondrial Functions.^1,3

Services

Cardiotoxicity is a common and serious side effect associated with cancer therapies, attracting considerable attention. Creative Biolabs offers CRO services to analyze the cardiotoxic effects of anticancer agents, especially from the perspective of mitochondrial function.

• Mitochondrial Function Valuation: Examine the impact of agents on the mitochondrial respiratory chain by assessing the oxygen consumption rate (OCR) and the utilization of amino acids.
• ATP Production Measurement: Employ fluorescent probe techniques to assess the impact of medications on mitochondrial ATP production capabilities, thus identifying alterations in energy metabolism.
• Analysis of Mitochondrial Membrane Potential: Evaluate the effects of drugs on mitochondrial membrane potential using JC-1 dye or other probes, which can indicate the functional integrity of mitochondria.
• Examination of Mitochondrial Structure: Apply electron microscopy or fluorescence imaging methods to detect drug-induced changes in mitochondrial structure and quantity, assessing potential damage to cardiac cells.
• Gene Expression Analysis: Utilize RNA sequencing or qPCR to identify modifications in gene expression associated with mitochondrial function, thereby investigating the molecular mechanisms contributing to drug-induced cardiotoxicity.
• Evaluation of Apoptosis and Survival Pathways: Identify apoptosis-related proteins (such as Caspase-3, Bcl-2, and Cytochrome c) through Western blotting or ELISA techniques. Additionally, by employing Annexin V/PI staining, assess the rates of apoptosis to gain insights into the effects of drugs on myocardial cell survival.

Fig.2 Mitochondrial Enrichment Detection System.^2,3

Case Study

In a recent project, our team used a high-throughput mitochondrial respiration analyzer to evaluate the activity of the mitochondrial respiratory chain in mouse neuronal samples. We constructed a mitochondrial function profile based on the oxygen consumption rate (OCR) and amino acid metabolism, analyzing the efficiency of mitochondrial respiration, ATP production, and redox status before and after treatment with anticancer drugs. By integrating various analytical methods such as flow cytometry and real-time fluorescence imaging, we were able to conduct a comprehensive and multidimensional assessment of mitochondrial physiological conditions.

During the data analysis phase, we employed artificial intelligence algorithms to conduct a full exploration of the experimental results, revealing significant changes in mitochondrial membrane potential following drug treatment. Ultimately, we produced a detailed report identifying key biomarkers related to mitochondrial function and provided recommendations for the design of subsequent experiments.

Anti-cancer drugs can occasionally harm mitochondria, leading to cardiotoxicity. By conducting a thorough analysis of mitochondrial function, Creative Biolabs can detect potential drug side effects and provide reliable data to support the safety evaluation of these drugs. We offer high-quality mitochondrial function analysis services to support your research in the development of cancer therapeutics. Feel free to contact us to discuss your project requirements and the specific details of mitochondrial function analysis.

References

1. Zong, Yao, et al. "Mitochondrial dysfunction: mechanisms and advances in therapy." Signal Transduction and Targeted Therapy 9.1 (2024): 124.
2. McLaughlin, Kelsey L., et al. "Novel approach to quantify mitochondrial content and intrinsic bioenergetic efficiency across organs." Scientific Reports 10.1 (2020): 17599.
3. Distributed under Open Access license CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use