Monocyte Activation Test
The Monocyte Activation Test (MAT) assay employs human blood to simulate the first stages of the human immune system, which is an in vitro assay designed to test parenteral drugs, biologics, and medical devices for all classifications of pyrogens, and it is an alternative to both the RPT and LAL tests. Moreover, it also offers a solution for testing products that prove to be problematic in other in vitro endotoxin tests, such as drugs that cause immunological reactions, detergents, some blood-derived products, drugs that affect body temperature regulation, and other products that are turbidimetric, strongly colored, or interfere with clotting. Creative Biolabs provides monocyte activation test service to bring sensitive and reliable detection of endotoxins and non-endotoxin pyrogens to a test.
MAT Workflow
Fig.1 The workflow of MAT.
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Step 1: The sample is incubated with peripheral blood mononuclear cells (PBMC) included in the MAT kit.
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Step 2: Toll-like receptors of the monocytes could detect any pyrogens that may be present in the sample preparation. If there are any, will launch the human immune system response which activates cytokine release.
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Step 3: A human IL-6 ELISA (included in the MAT kit) is employed as a readout to quantify the concentration of released cytokines in the product preparation.
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Step 4: Making use of the LPS standard curve, the measured cytokine concentration is then converted to the endotoxin equivalent units/mL.
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Human-specific, in-vitro & most sensitive
MAT is the only endotoxin or pyrogen assay in vitro, which reflects the human immune system and with an LoD of 0.004 EU/mL which is the most sensitive.
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Detects broadest range of pyrogens
Only MAT can detect all pyrogen classifications including those deriving from gram positive and gram-negative bacteria, as well as yeasts, molds and viruses.
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Optimized for the most product categories
MAT is successful in testing all products, including medical devices, therapeutics, pharmaceuticals & biologics.
Advantages
Experts at Creative Biolabs are happy to develop custom MAT to satisfy your testing objectives. You could purchase the corresponding MAT products or send samples to seek our one-stop endotoxin testing service. For more detailed information, please feel free to contact us or directly send us an inquiry.
Reference
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Dullah, E.C.; et al. Current trends in endotoxin detection and analysis of endotoxin-protein interactions. Crit Rev Biotechnol. 2017, 37(2): 251-261.
For Research Use Only | Not For Clinical Use
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