NRK-52E-based Cytotoxicity Safety Screening Service

Safeguarding with Precision: NRK-52E Cytotoxicity Screening

Creative Biolabs specializes in providing comprehensive In Vitro ADME services, tailored specifically to support the development of immunotherapy.

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We proudly offer a specialized NRK-52E-based cytotoxicity safety screening service. This service is meticulously designed to assess the safety profiles of compounds and products, ensuring they meet stringent safety standards before advancing further in development or clinical applications. Through innovative methodologies and expert analysis, we provide reliable data crucial for informed decision-making in biomedical research and pharmaceutical development.

Characteristics of NRK-52E Cell:

• Cell Origin: NRK-52E cells are derived from normal rat kidney epithelial tissue.
• Cell Type: They are epithelial cells, specifically renal proximal tubular cells.
• Morphology: They maintain typical epithelial morphology, forming monolayers with a polygonal or cobblestone-like appearance.
• Physiological Relevance: These cells retain functional characteristics similar to renal epithelial cells, making them suitable for studying nephrotoxicity and renal safety profiles.

Our method utilizes NRK-52E cells for functional cytotoxicity screening, employing Beetle luciferin + ATP as the substrate and Tamoxifen as the control inhibitor. Luminescence is employed as the detection method, ensuring sensitive and accurate measurement of cytotoxic responses in this renal cell model.

Assay Information:

Workflow

Our rigorous technical procedures ensure thorough evaluation of compound safety profiles in renal cells, facilitating the advancement of safe and effective therapeutic innovations.

1. Cell Culture Preparation

NRK-52E cells are cultured under optimized conditions to ensure adherence and viability.

2. Treatment and Incubation

Cells are exposed to varying concentrations of test compounds, including Tamoxifen as a control inhibitor.

3. Luminescence Assay

Beetle luciferin + ATP substrate is added to initiate luminescence production in treated cells.

4. Signal Detection

Luminescent signals are quantified using appropriate detection instruments to measure cytotoxicity levels accurately.

5. Data Analysis

Results are meticulously analyzed to determine the impact of test compounds on NRK-52E cell viability and functionality.

6. Reporting and Interpretation

Comprehensive reports are generated, summarizing findings and providing actionable insights for further research and development strategies.

Published Data

This study demonstrates that doxorubicin complements αklotho expression in NRK-52E cells. The facts imply a big upregulation of αklotho ranges in NRK-52E cells following exposure to doxorubicin, a commonly used chemotherapeutic agent. This upregulation shows a functionality-protecting or adaptive mobile reaction to the cytotoxic outcomes of doxorubicin. Understanding the modulation of αklotho expression in response to cytotoxic sellers can provide precious insights into the mechanisms of renal cellular safety and the development of strategies to mitigate nephrotoxicity in recovery programs. Further studies are warranted to provide an explanation for the best pathways and their implications for reinforcing kidney health during most cancer remedies.

Fig.1 Doxorubicin induces αklotho expression in NRK-52E cells.¹

Are you looking to evaluate the safety of compounds, on kidney cells for developing medications? Do you need help understanding how certain agents affect kidney cell health and their toxic effects? Our research can shed light on nephrotoxicity mechanisms and drug-induced kidney injuries leading to treatment options. For the NRK 52E cells we use epithelial tissues accurately ensuring that our toxicity evaluations reflect real-world scenarios. By employing Beetle luciferin + ATP substrate and Tamoxifen as a control inhibitor we guarantee precise measurements through luminescence detection. Trust our team for data analysis and interpretation.

For comprehensive cytotoxicity safety screening using NRK 52E cells choose Biolabs. We are committed to excellence and innovative methods that guarantee an assessment of compound safety profiles for advancing therapeutic research and meeting regulatory requirements. Reach out to us to learn more, about our cytotoxicity safety screening services.

Reference

1. Münz, Sina, et al. "Impact of cytotoxic agents or apoptosis stimulants on αklotho in MDCK, NRK-52E and HK2 kidney cells." Aging (Albany NY) 14.18 (2022): 7282.

For Research Use Only | Not For Clinical Use