Parallel Artificial Membrane Permeability Determination Service
Precision Permeability for Superior Drug Development
Creative Biolabs offers cutting-edge In Vitro ADME services meticulously tailored to enhance cancer immunotherapy research, ensuring optimized drug absorption, distribution, metabolism, and excretion profiles.
Parallel artificial membrane permeability assay (PAMPA) is an essential in vitro method to assess the passive permeability of drug candidates. This assay mimics the lipid bilayer of biological cell membranes, providing essential insights into a compound's ability to cross cell membranes. PAMPA is highly valuable in early drug development, allowing for rapid screening and identification of compounds with desirable permeability characteristics, thereby facilitating the selection of candidates with optimal bioavailability.
Creative Biolabs is proud to offer top-tier parallel artificial membrane permeability determination services. Leveraging advanced technologies and extensive expertise, we deliver precise and reliable permeability data that is vital for optimizing drug formulations and enhancing therapeutic efficacy. We have well-thought-out designs for our PAMPA services intended to achieve a complete complement of permeability profiles using robust, reliable methods:
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PAMPA Setup and Execution:
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Artificial Membrane Preparation: We use an artificial liposome membrane that is lipid-rich and closely mimics the properties of a biological cell membrane for accurate passive permeability simulation.
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Assay Plate Setup: Compounds are added to donor wells, and incubated to permeate through the artificial membrane into acceptor wells which represents drug absorption.
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Incubation Parameters: Temperature-controlled, optimized incubation of experiments.
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Selection Criteria of Different Types of PAMPA Models
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Traditional PAMPA
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Ideal for initial screening of passive permeability of small molecules.
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Gastrointestinal PAMPA (GIP-AMPA)
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Select this model for drugs intended for oral administration to simulate gastrointestinal absorption.
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Blood-Brain Barrier PAMPA (BBB-PAMPA)
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Select this model to determine the capability of drugs aimed at the central nervous system to penetrate the blood-brain barrier.
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Skin PAMPA
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Use this model for transdermal or topical drugs to evaluate skin permeability.
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Artificial Membrane Chromatography PAMPA (AMC-PAMPA)
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Provides more detailed permeability data, useful for complex compounds and formulations.
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After the incubation period, the acceptor well samples undergo HPLC-MS/MS analysis. Through this advanced detection method, precise measurements of compound concentrations are obtained with high sensitivity and accuracy, ultimately producing accurate permeability data based on peak area response.
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Data Analysis and Reporting
We perform quantitative assessments of compound permeability, providing detailed insights into their potential bioavailability. Our comprehensive reports include raw data, analyzed results, and expert interpretations, empowering informed decision-making throughout the drug development process.
Advantages
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High Throughput: PAMPA enables the simultaneous assessment of multiple compounds, accelerating the screening process and saving valuable time.
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Reliable and Reproducible: Our rigorous methodologies ensure consistent and reproducible results, providing confidence in the data and supporting robust decision-making.
FAQ
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Which PAMPA model should I choose for my study?
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Oral Drugs: GIPAMPA.
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CNS Drugs: BBB-PAMPA.
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Transdermal/Topical Drugs: Skin PAMPA.
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Initial Screening: Traditional PAMPA.
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Detailed Analysis: AMC-PAMPA.
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What detection methods are used in PAMPA?
Typically, HPLC-MS/MS is used to detect and quantify the compounds that have permeated through the membrane by measuring the peak area response.
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What are the advantages of using PAMPA?
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High Throughput: Allows simultaneous assessment of multiple compounds.
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Cost-Effective: More affordable than complex in vivo permeability studies.
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Reproducible Results: Provides consistent and reliable data.
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Rapid Screening: Enables quick identification of promising drug candidates.
Creative Biolabs' PAMPA service delivers precise and reliable permeability analysis, essential for the successful development of drug candidates. Our expert team and state-of-the-art methodologies ensure comprehensive data to optimize drug formulations and enhance therapeutic efficacy. Contact us today to discover how our specialized PAMPA services can accelerate your research and development efforts, driving your projects toward successful outcomes.
For Research Use Only | Not For Clinical Use
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