PathFinder™ PD-1[Biotinylated]:PD-L2 Inhibitor Screening Colorimetric Assay Kit (CAT#: IOK-02P089)

After binding to PD-L2 ligand, the cell signal transduction through PD-1 receptor; weakens the immune response, and is used by tumors and viruses. PD-; 1 [Biotinylation]: PD-L2 inhibitor screening colorimetric analysis kit for screening; inhibitor of PD-1 receptor binding to PD-L2 ligand. The key to this kit is that biotin-labeled PD-1 has high affinity. Streptavidin-HRP. The determination only requires a few simple steps on the microtiter plate. The first one is; PD-L2 is coated on a 96-well plate. Next, PD-1 was incubated with PD-L2 on the plate. Finally, the plate is treated with streptavidin-HRP, and then a colorimetric HRP substrate is added; the substrate produces color, and then the color can be measured using a UV/Vis spectrophotometer; a microplate reader.

Cell signaling through the PD-1 receptor upon binding the PD-L2 ligand; attenuates immune responses and is exploited by both tumors and viruses. The PD-; 1[Biotinylated]:PD-L2 Inhibitor Screening Colorimetric Assay Kit is designed for screening; inhibitors of PD-1 receptor binding to PD-L2 ligand. The key to this kit is the high affinity of biotin-labeled PD-1 for; streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First,; PD-L2 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L2 on the plate. Finally, the; plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate; substrate to produce color, which can then be measured using a UV/Vis spectrophotometer; microplate reader.

This kit is most useful for screening for inhibitors of PD-L2.

Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L and Programmed Cell Death 2 Ligand 2, PDL2, PD-L2, CD273, PDCDL1G2, B7-DC